Central Role of Manganese in Regulation of Stress Responses, Physiology, and Metabolism in Streptococcus pneumoniae

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Title Central Role of Manganese in Regulation of Stress Responses, Physiology, and Metabolism in Streptococcus pneumoniae
Author Ogunniyi, Abiodun D.; Mahdi, Layla K.; Jennings, Michael Paul; McEwan, Alastair G.; McDevitt, Christopher A.; Hoek, Mark B. Van der; Bagley, Christopher J.; Hoffmann, Peter; Gould, Katherine A.; Paton, James C.
Journal Name Journal of Bacteriology
Year Published 2010
Place of publication United States
Publisher American Society for Microbiology
Abstract The importance of Mn2+ for pneumococcal physiology and virulence has been studied extensively. However, the specific cellular role(s) for which Mn2+ is required are yet to be fully elucidated. Here, we analyzed the effect of Mn2+ limitation on the transcriptome and proteome of Streptococcus pneumoniae D39. This was carried out by comparing a deletion mutant lacking the solute binding protein of the high-affinity Mn2+ transporter, pneumococcal surface antigen A (PsaA), with its isogenic wild-type counterpart. We provide clear evidence for the Mn2+-dependent regulation of the expression of oxidative-stress-response enzymes SpxB and Mn2+-SodA and virulence-associated genes pcpA and prtA. We also demonstrate the upregulation of at least one oxidative- and nitrosative-stress-response gene cluster, comprising adhC, nmlR, and czcD, in response to Mn2+ stress. A significant increase in 6-phosphogluconate dehydrogenase activity in the psaA mutant grown under Mn2+-replete conditions and upregulation of an oligopeptide ABC permease (AppDCBA) were also observed. Together, the results of transcriptomic and proteomic analyses provided evidence for Mn2+ having a central role in activating or stimulating enzymes involved in central carbon and general metabolism. Our results also highlight the importance of high-affinity Mn2+ transport by PsaA in pneumococcal competence, physiology, and metabolism and elucidate mechanisms underlying the response to Mn2+ stress.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1128/JB.00064-10
Volume 192
Issue Number 17
Page from 4489
Page to 4497
ISSN 0021-9193
Date Accessioned 2011-01-24
Language en_AU
Research Centre Institute for Glycomics
Faculty Griffith Health Faculty
Subject Medical Microbiology
URI http://hdl.handle.net/10072/35816
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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