Crystallization and preliminary X-ray crystallographic analysis of zebrafish prototype galectin Drgal1-L2

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Title Crystallization and preliminary X-ray crystallographic analysis of zebrafish prototype galectin Drgal1-L2
Author Scott, Stacy; Cozier, Matthew Oliver; Dubar, Pauline Dominique Isabelle; Ramakrishna, Manasa; Scott, Ken; Blanchard, Helen
Journal Name Acta Crystallographica Section F
Editor Einspahr, Weiss
Year Published 2010
Place of publication United States
Publisher Wiley-Blackwell Publishing, Inc.
Abstract Zebrafish (Danio rerio) are an important developmental and embryological model given the optical clarity of embryos and larvae that permit real-time viewing of developing pathologies. More recently, a broader scope for these vertebrates to model a range of human diseases, including some cancers, has been indicated. Zebrafish Drgal1-L2 has been identified as an orthologue of mammalian galectin-1 that is a carbohydrate-binding protein exhibiting β-galactoside binding specificity and which is over-expressed by many aggressive human cancers. This study describes the cloning, expression in Escherichia coli, purification and crystallisation of recombinant Drgal1-L2 protein in the presence of lactose (ligand). X-ray diffraction data from these novel crystals of zebrafish Drgal1-L2 were collected to a resolution of 1.5 Å using a synchrotron radiation source, enabling their characterisation.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1107/S1744309110042272
Volume F66
Issue Number 12
Page from 1647
Page to 1651
ISSN 1744-3091
Date Accessioned 2011-01-27
Date Available 2011-02-23T08:56:23Z
Language en_AU
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Biological Physics
URI http://hdl.handle.net/10072/36511
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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