Galectin inhibitory disaccharides promote tumour immunity in a breast cancer model

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Title Galectin inhibitory disaccharides promote tumour immunity in a breast cancer model
Author Stannard, Kimberley Ann; Collins, Patrick; Ito, Koichi; Sullivan, Emily May; Scott, Stacy; Gabutero, Elwyn Reg; Grice, Darren; Low, Pauline; Nilsson, Ulf. J.; Leffler, Hakon; Blanchard, Helen; Ralph, Stephen John
Journal Name Cancer Letters
Year Published 2010
Place of publication Ireland
Publisher Elsevier
Abstract High level galectin-1 expression results in cancer cell evasion of the immune response, increased tumour survival and aggressive metastases. Using a galectin-1 polyclonal antibody, high levels of galectin-1 protein were shown to be expressed by breast cancer cells established from FVB/N MMTV-c-neu mice as well as by the B16F10 melanoma cell line. In mixed lymphocyte cultures using tumour cells as antigenic stimulators, addition of recombinant galectin-1 dose-dependently inhibited lymphocyte production. Disaccharides were identified that inhibited galectin-1 function and increased growth and activation of CD8+ CTL's killing cancer cells. X-ray crystallographic structures of human galectin-1 in complex with inhibitory disaccharides revealed their mode of binding. Combining galectin-blocking carbohydrates as adjuvants with vaccine immunotherapy in vivo to promote immune responses significantly decreased tumour progression and improved the outcomes for tumour challenged mice. This is the first report showing that suitably selected galectin-1 blocking disaccharides will act as adjuvants promoting vaccine stimulated immune responses against tumours in vivo.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1016/j.canlet.2010.08.005
Volume 299
Issue Number 2
Page from 95
Page to 110
ISSN 0304-3835
Date Accessioned 2010-11-26
Date Available 2011-03-07T08:52:55Z
Language en_AU
Research Centre Griffith Health Institute; Institute for Glycomics; Molecular Basis of Disease
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Oncology and Carcinogenesis
URI http://hdl.handle.net/10072/36847
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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