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Moraxella catarrhalis Lgt2, a galactosyltransferase with broad acceptor substrate specificity

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Title Moraxella catarrhalis Lgt2, a galactosyltransferase with broad acceptor substrate specificity
Author Faglin, Isabelle; Wilson, Jenny; Tiralongo, Joe; Peak, Ian
Journal Name Carbohydrate Research
Year Published 2010
Place of publication United Kingdom
Publisher Elsevier
Abstract The genetic basis of lipo-oligosaccharide (LOS) biosynthesis for the bacterium Moraxella catarrhalis has been elucidated and functions suggested for each of the glycosyltransferases. In this study we have expressed and characterised one of these enzymes, the putative galactosyltransferase Lgt2B/C. The lgt2B/C gene was amplified from M. catarrhalis, expressed in Escherichia coli, and Lgt2B/C was purified. Analysis of its glycosyltransferase catalytic activity ascertained the pH and temperature optima. The donor specificity and acceptor specificity were examined and they showed that Lgt2B/C is a galactosyltransferase with relatively broad acceptor specificity with optimal activity in the presence of exogenous Mg2+.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1016/j.carres.2010.07.042
Volume 345
Issue Number 15
Page from 2151
Page to 2156
ISSN 0008-6215
Date Accessioned 2010-11-26
Date Available 2012-02-10T02:23:08Z
Language en_US
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Organic Chemistry
URI http://hdl.handle.net/10072/36921
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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