Effect of 830nm laser phototherapy on olfactory neuronal ensheathing (progenitor-like) cells grown in vitro on bioscaffolds

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Title Effect of 830nm laser phototherapy on olfactory neuronal ensheathing (progenitor-like) cells grown in vitro on bioscaffolds
Author Laakso, Liisa; Renno, Ana Claudia; McDonnell, Ann
Publication Title 8th International Congress of the World Association for Laser Therapy - WALT
Editor Jan Magnus Bjordal
Year Published 2010
Place of publication Norway
Publisher World Association for Laser Therapy
Abstract Background and Objective: Our group is investigating the effect of laser phototherapy on a range of cell lines seeded onto novel bioscaffolds as a potential method for assisting in tissue repair and healing. Our aim was to analyse the cell proliferation and growth characteristics of the olfactory neuronal ensheathing cells on the chosen bioscaffolds. Methods: Olfactory lamina propria ensheathing (OLF442) cells were cultured, seeded and grown on two novel scaffolds: (1) fully crystallised bioactive glass-ceramic, and (2) modified spongin collagen matrix. Twenty-four hours after seeding of scaffolds, cells were irradiation with a single exposure of laser (830 nm continuous, 30 mW, 10 J/cm2; Smart Laser Medilase, NSW, Australia). Cell proliferation was calculated with spectrophotometry. Scanning electron micrography was utilised to visualise cell growth, migration patterns, and morphology of cells growing on the three-dimensional scaffolds. Results: Laser irradiation produced a 29% mean reduction in OLF442 cell growth on the glass–ceramic scaffold discs (mean absorbance +/- SD = 0.192+/-0.002) compared to control (non-irradiated) discs (mean absorbance +/- SD = 0.22+/-0.002). There was a 43% mean increase in OLF442 cell proliferation on the modified collagen scaffolds compared to non-irradiated control scaffolds. Conclusion: Both scaffolds were capable of supporting OLF442 cell growth. There was a stimulatory effect of phototherapy on OLF442 cells in collagen derived scaffolds, and inhibitory effect of the same laser attributes on a glass-ceramic scaffold. The results support the potential use of laser for promoting tissue healing using bioscaffolds but the type of scaffold may affect the laser-mediated outcomes.
Peer Reviewed No
Published Yes
Publisher URI http://www.walt.nu/
ISBN 978-887587-597-8
Conference name 8th International Congress of the World Association for Laser Therapy
Location Bergen Norway
Date From 2010-09-25
Date To 2010-09-28
URI http://hdl.handle.net/10072/37297
Date Accessioned 2011-02-14
Date Available 2011-03-16T07:56:45Z
Language en_AU
Research Centre Griffith Health Institute; Centre for Musculoskeletal Research
Faculty Griffith Health Faculty
Subject Complementary and Alternative Medicine
Publication Type Conference Publications (Extract Paper)
Publication Type Code e3

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