RNAi Screen Indicates Widespread Biological Function for Human Natural Antisense Transcripts

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Title RNAi Screen Indicates Widespread Biological Function for Human Natural Antisense Transcripts
Author Faghihi, Mohammad Ali; Kocerha, Jannet; Modarresi, Farzaneh; Engström, Pär G.; Chalk, Alistair Morgan; Brothers, Shaun P.; Koesema, Eric; St. Laurent III, Georges; Wahlestedt, Claes
Journal Name PLoS One
Year Published 2010
Place of publication United States
Publisher Public Library of Science
Abstract Natural antisense transcripts represent a class of regulatory RNA molecules, which are characterized by their complementary sequence to another RNA transcript. Extensive sequencing efforts suggest that natural antisense transcripts are prevalent throughout the mammalian genome; however, their biological significance has not been well defined. We performed a loss-of-function RNA interference (RNAi) screen, which targeted 797 evolutionary conserved antisense transcripts, and found evidence for a regulatory role for a number of natural antisense transcripts. Specifically, we found that natural antisense transcripts for CCPG1 and RAPGEF3 may functionally disrupt signaling pathways and corresponding biological phenotypes, such as cell viability, either independently or in parallel with the corresponding sense transcript. Our results show that the large-scale siRNA screen can be applied to evaluate natural antisense transcript modulation of fundamental cellular events.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1371/journal.pone.0013177
Copyright Statement Copyright 2010 Faghih et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
Volume 5
Issue Number 10
Page from 1
Page to 10
ISSN 1932-6203
Date Accessioned 2011-01-10
Language en_US
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Genetics
URI http://hdl.handle.net/10072/37363
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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