Low doses of killed parasite in CpG elicit vigorous CD4+T cell responses against blood-stage malaria in mice

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Title Low doses of killed parasite in CpG elicit vigorous CD4+T cell responses against blood-stage malaria in mice
Author Pinzon-Charry, Alberto; McPhun, Virginia; Kienzle, Vivian; Hirunpetcharat, Chakrit; Engwerda, Christian; McCarthy, James; Good, Michael Francis
Journal Name Journal of Clinical Investigation
Year Published 2010
Place of publication America
Publisher American Society for Clinical Investigation
Abstract Development of a vaccine that targets blood-stage malaria parasites is imperative if we are to sustainably reduce the morbidity and mortality caused by this infection. Such a vaccine should elicit long-lasting immune responses against conserved determinants in the parasite population. Most blood-stage vaccines, however, induce protective antibodies against surface antigens, which tend to be polymorphic. Cell-mediated responses, on the other hand, offer the theoretical advantage of targeting internal antigens that are more likely to be conserved. Nonetheless, few of the current blood-stage vaccine candidates are able to harness vigorous T cell immunity. Here, we present what we believe to be a novel blood-stage whole-organism vaccine that, by combining low doses of killed parasite with CpG-oligodeoxynucleotide (CpG-ODN) adjuvant, was able to elicit strong and cross-reactive T cell responses in mice. Our data demonstrate that immunization of mice with 1,000 killed parasites in CpG-ODN engendered durable and cross-strain protection by inducing a vigorous response that was dependent on CD4+ T cells, IFN-gamma, and nitric oxide. If applicable to humans, this approach should facilitate the generation of robust, cross-reactive T cell responses against malaria as well as antigen availability for vaccine manufacture
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1172/JCI39222
Volume 120
Issue Number 8
Page from 2967
Page to 2978
ISSN 2967–2978
Date Accessioned 2011-03-29
Language en_AU
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Infectious Diseases
URI http://hdl.handle.net/10072/38010
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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