The in vitro effects of LASER on human mammary epithelial and human breast cancer cell lines

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Title The in vitro effects of LASER on human mammary epithelial and human breast cancer cell lines
Author Laakso, Liisa; Powell, Katie; McDonnell, Ann; Low, Pauline; Ralph, Stephen John
Publication Title 8th Australasian Lymphology Association Conference: Reflections on the past, Inspirations for the future
Editor L. Laakso
Year Published 2010
Place of publication Australia
Publisher Australasian Lymphology Association
Abstract Background: Despite limited safety data, Low Level Laser Therapy (LLLT) is used in the clinical treatment of breast cancer-related lymphoedema (BCRL). Objective: We aimed to compare the cell proliferative effects of a range of doses of LLLT at wavelengths of 780, 830 and 904 nm on human breast and immortalised human mammary epithelial cell lines in vitro. Materials and Methods: Human breast adenocarcinoma (MCF-7), human melanoma cell line (MDA-MB-435S/M14) and immortalised human mammary epithelial (SVCT and Bre80hTERT) cell lines were irradiated with a single exposure of LASER at 0.5, 1, 2, 3, 4, 10 and 12 J/cm2 (=780 nm) and 0.5, 1, 2, 3, 4, 10 and 15 J/cm2 (=830 and 904 nm). MCF-7 cells were further irradiated with two and three exposures of all wavelengths. Cell proliferation was assessed 24 hours after irradiation. Results: SVCT cell proliferation significantly increased after exposure to a range of doses at 780 and 904 nm irradiation. MDA-MB-435S and Bre80hTERT cell lines showed negligible effects to LASER exposure. MCF-7 cells irradiated with 780 nm LASER demonstrated an increasing dose response relationship after one exposure and a decreasing dose response relationship after three exposures. MCF-7 cells irradiated with 904 nm LASER demonstrated a decreasing dose response relationship after two and three exposures. Conclusion: Although certain LASER doses increased MCF-7 cell proliferation, multiple exposures had no effect or a decreasing effect on dose response relationships. Before a definitive conclusion can be made regarding LASER safety for BCRL, further in vivo research is necessary.
Peer Reviewed Yes
Published Yes
Publisher URI http://lymphology.asn.au/new/index.php
Conference name 8th Australasian Lymphology Association Conference
Location Melbourne, Australia
Date From 2010-05-27
Date To 2010-05-29
URI http://hdl.handle.net/10072/38985
Date Accessioned 2011-02-14
Language en_AU
Research Centre Griffith Health Institute; Centre for Musculoskeletal Research; Molecular Basis of Disease
Faculty Griffith Health Faculty
Subject Medical and Health Sciences
Publication Type Conference Publications (Full Written Paper - Refereed)
Publication Type Code e1

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