The in vitro effects of LASER on human mammary epithelial and human breast cancer cell lines

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Title The in vitro effects of LASER on human mammary epithelial and human breast cancer cell lines
Author Laakso, Liisa; Powell, Katie; McDonnell, Ann; Low, Pauline; Ralph, Stephen John
Publication Title 8th Australasian Lymphology Association Conference: Reflections on the past, Inspirations for the future
Editor L. Laakso
Year Published 2010
Place of publication Australia
Publisher Australasian Lymphology Association
Abstract Background: Despite limited safety data, Low Level Laser Therapy (LLLT) is used in the clinical treatment of breast cancer-related lymphoedema (BCRL). Objective: We aimed to compare the cell proliferative effects of a range of doses of LLLT at wavelengths of 780, 830 and 904 nm on human breast and immortalised human mammary epithelial cell lines in vitro. Materials and Methods: Human breast adenocarcinoma (MCF-7), human melanoma cell line (MDA-MB-435S/M14) and immortalised human mammary epithelial (SVCT and Bre80hTERT) cell lines were irradiated with a single exposure of LASER at 0.5, 1, 2, 3, 4, 10 and 12 J/cm2 (=780 nm) and 0.5, 1, 2, 3, 4, 10 and 15 J/cm2 (=830 and 904 nm). MCF-7 cells were further irradiated with two and three exposures of all wavelengths. Cell proliferation was assessed 24 hours after irradiation. Results: SVCT cell proliferation significantly increased after exposure to a range of doses at 780 and 904 nm irradiation. MDA-MB-435S and Bre80hTERT cell lines showed negligible effects to LASER exposure. MCF-7 cells irradiated with 780 nm LASER demonstrated an increasing dose response relationship after one exposure and a decreasing dose response relationship after three exposures. MCF-7 cells irradiated with 904 nm LASER demonstrated a decreasing dose response relationship after two and three exposures. Conclusion: Although certain LASER doses increased MCF-7 cell proliferation, multiple exposures had no effect or a decreasing effect on dose response relationships. Before a definitive conclusion can be made regarding LASER safety for BCRL, further in vivo research is necessary.
Peer Reviewed Yes
Published Yes
Publisher URI
Conference name 8th Australasian Lymphology Association Conference
Location Melbourne, Australia
Date From 2010-05-27
Date To 2010-05-29
Date Accessioned 2011-02-14
Language en_AU
Research Centre Centre for Musculoskeletal Research; Menzies Health Institute Qld; Molecular Basis of Disease
Faculty Griffith Health Faculty
Subject Medical and Health Sciences
Publication Type Conference Publications (Full Written Paper - Refereed)
Publication Type Code e1

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