A novel binding assay to assess specificity of monoclonal antibodies

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Title A novel binding assay to assess specificity of monoclonal antibodies
Author Bettadapura, Jayaram; Warren, Hilary S.
Journal Name Journal of Immunological Methods
Editor M.C. Nussenzweig
Year Published 2005
Place of publication Elsevier
Publisher Association of medical Laboratory Immunologists
Abstract Dynabeads® TALON™ are uniform superparamagnetic polystyrene beads of 1μm diameter that bind 6-His-tagged recombinant proteins through Co++-affinity binding, and are normally used for protein purification. We have used these beads to bind 6-His-rNKp30 and 6-His-rNKp46 to use as a matrix for evaluating NKp30 and NKp46 mAb submitted to the 8th International Human Leukocyte Differentiation Antigen Workshop. We show that recombinant protein coated beads are an effective tool to evaluate the specificity and epitope reactivity of mAb.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1016/j.jim.2005.07.011
Volume 305
Page from 33
Page to 38
ISSN 0022-1759
Date Accessioned 2011-03-17
Language en_AU
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Innate Immunity
URI http://hdl.handle.net/10072/39313
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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