A novel binding assay to assess specificity of monoclonal antibodies
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| Title | A novel binding assay to assess specificity of monoclonal antibodies |
|---|---|
| Author | Bettadapura, Jayaram; Warren, Hilary S. |
| Journal Name | Journal of Immunological Methods |
| Editor | M.C. Nussenzweig |
| Year Published | 2005 |
| Place of publication | Elsevier |
| Publisher | Association of medical Laboratory Immunologists |
| Abstract | Dynabeads® TALON™ are uniform superparamagnetic polystyrene beads of 1μm diameter that bind 6-His-tagged recombinant proteins through Co++-affinity binding, and are normally used for protein purification. We have used these beads to bind 6-His-rNKp30 and 6-His-rNKp46 to use as a matrix for evaluating NKp30 and NKp46 mAb submitted to the 8th International Human Leukocyte Differentiation Antigen Workshop. We show that recombinant protein coated beads are an effective tool to evaluate the specificity and epitope reactivity of mAb. |
| Peer Reviewed | Yes |
| Published | Yes |
| Alternative URI | http://dx.doi.org/10.1016/j.jim.2005.07.011 |
| Volume | 305 |
| Page from | 33 |
| Page to | 38 |
| ISSN | 0022-1759 |
| Date Accessioned | 2011-03-17 |
| Date Available | 2011-06-29T08:13:48Z |
| Language | en_AU |
| Faculty | Faculty of Science, Environment, Engineering and Technology |
| Subject | Innate Immunity |
| URI | http://hdl.handle.net/10072/39313 |
| Publication Type | Journal Articles (Refereed Article) |
| Publication Type Code | c1x |
Please use this identifier to cite this record: http://hdl.handle.net/10072/39313
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