Real-time RT-PCR for norovirus screening in shellfish

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Title Real-time RT-PCR for norovirus screening in shellfish
Author Loisy, F.; Atmar, R.L.; Guillon, Patrice Michel Guy; Cann, P. Le; Pommepuy, M.; Guyader, F.S. Le
Journal Name Journal of virological methods
Year Published 2005
Place of publication Netherlands
Publisher Elsevier
Abstract Real-time RT-PCR, combining amplification and detection of virus-specific amplicons, is a promising tool for norovirus detection in environmental or food samples such as shellfish. We developed a real-time RT-PCR assay based on one-step detection using single primer sets and probes for norovirus genogroups I and II. Seventy and seven RT-PCR units of genogroup I and II reference norovirus strains, respectively, were detected in artificially contaminated oysters. Validation of the new method on 150 archived naturally contaminated shellfish confirmed the utility of the genogroup II primer set to detect a large range of different strains circulating in France since 1995, but genogroup I strains were detected infrequently.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1016/j.jviromet.2004.08.023
Volume 123
Issue Number 1
Page from 1
Page to 7
ISSN 0166-0934
Date Accessioned 2010-07-26
Date Available 2011-07-09T02:31:08Z
Language en_AU
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Virology
URI http://hdl.handle.net/10072/39466
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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