Show simple item record

dc.contributor.authorKing, Nathan P
dc.contributor.authorBeatson, Scott A
dc.contributor.authorTotsika, Makrina
dc.contributor.authorUlett, Glen C
dc.contributor.authorAlm, Richard A
dc.contributor.authorManning, Paul A
dc.contributor.authorSchembri, Mark A
dc.date.accessioned2017-05-03T15:29:33Z
dc.date.available2017-05-03T15:29:33Z
dc.date.issued2011
dc.date.modified2011-08-16T07:29:09Z
dc.identifier.issn1350-0872
dc.identifier.doi10.1099/mic.0.047639-0
dc.identifier.urihttp://hdl.handle.net/10072/40063
dc.description.abstractStaphylococcus saprophyticus is an important cause of urinary tract infection (UTI), particularly among young women, and is second only to uropathogenic Escherichia coli as the most frequent cause of UTI. The molecular mechanisms of urinary tract colonization by S. saprophyticus remain poorly understood. We have identified a novel 6.84 kb plasmid-located adhesin-encoding gene in S. saprophyticus strain MS1146 which we have termed uro-adherence factor B (uafB). UafB is a glycosylated serine-rich repeat protein that is expressed on the surface of S. saprophyticus MS1146. UafB also functions as a major cell surface hydrophobicity factor. To characterize the role of UafB we generated an isogenic uafB mutant in S. saprophyticus MS1146 by interruption with a group II intron. The uafB mutant had a significantly reduced ability to bind to fibronectin and fibrinogen. Furthermore, we show that a recombinant protein containing the putative binding domain of UafB binds specifically to fibronectin and fibrinogen. UafB was not involved in adhesion in a mouse model of UTI; however, we observed a striking UafB-mediated adhesion phenotype to human uroepithelial cells. We have also identified genes homologous to uafB in other staphylococci which, like uafB, appear to be located on transposable elements. Thus, our data indicate that UafB is a novel adhesin of S. saprophyticus that contributes to cell surface hydrophobicity, mediates adhesion to fibronectin and fibrinogen, and exhibits tropism for human uroepithelial cells.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSociety for General Microbiology
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationY
dc.relation.ispartofpagefrom1161
dc.relation.ispartofpageto1175
dc.relation.ispartofissue4
dc.relation.ispartofjournalMicrobiology
dc.relation.ispartofvolume157
dc.rights.retentionY
dc.subject.fieldofresearchMedical bacteriology
dc.subject.fieldofresearchcode320701
dc.titleUafB is a serine-rich repeat adhesin of Staphylococcus saprophyticus that mediates binding to fibronectin, fibrinogen and human uroepithelial cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2011
gro.hasfulltextNo Full Text
gro.griffith.authorUlett, Glen C.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record