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dc.contributor.authorBoerngen, Kirsten
dc.contributor.authorBattle, Andrew R
dc.contributor.authorMoeker, Nina
dc.contributor.authorMorbach, Susanne
dc.contributor.authorMarin, Kay
dc.contributor.authorMartinac, Boris
dc.contributor.authorKraemer, Reinhard
dc.date.accessioned2017-05-03T16:04:28Z
dc.date.available2017-05-03T16:04:28Z
dc.date.issued2010
dc.date.modified2011-08-19T06:42:58Z
dc.identifier.issn0005-2736
dc.identifier.doi10.1016/j.bbamem.2010.06.022
dc.identifier.urihttp://hdl.handle.net/10072/40107
dc.description.abstractBased on sequence similarity, the mscCG gene product of Corynebacterium glutamicum belongs to the family of MscS-type mechanosensitive channels. In order to investigate the physiological significance of MscCG in response to osmotic shifts in detail, we studied its properties using both patch-clamp techniques and betaine efflux kinetics. After heterologous expression in an Escherichia coli strain devoid of mechanosensitive channels, in patch-clamp analysis of giant E. coli spheroplasts MscCG showed the typical pressure dependent gating behavior of a stretch-activated channel with a current/voltage dependence indicating a strongly rectifying behavior. Apart from that, MscCG is characterized by significant functional differences with respect to conductance, ion selectivity and desensitation behavior as compared to MscS from E. coli. Deletion and complementation studies in C. glutamicum showed a significant contribution of MscCG to betaine efflux in response to hypoosmotic conditions. A detailed analysis of concomitant betaine uptake (by the betaine transporter BetP) and efflux (by MscCG) under hyperosmotic conditions indicates that MscCG may act in osmoregulation in C. glutamicum by fine-tuning the steady state concentration of compatible solutes in the cytoplasm which are accumulated in response to hyperosmotic stress.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom2141
dc.relation.ispartofpageto2149
dc.relation.ispartofissue11
dc.relation.ispartofjournalBiochimica et Biophysica Acta (BBA) - Biomembranes
dc.relation.ispartofvolume1798
dc.rights.retentionY
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchStructural biology (incl. macromolecular modelling)
dc.subject.fieldofresearchOther biological sciences
dc.subject.fieldofresearchChemical engineering
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode310112
dc.subject.fieldofresearchcode3199
dc.subject.fieldofresearchcode4004
dc.titleThe properties and contribution of the Corynebacterium glutamicum MscS variant to fine-tuning of osmotic adaptation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2010
gro.hasfulltextNo Full Text
gro.griffith.authorBattle, Andrew


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