Glucuronide-sulfate diconjugate as a novel metabolite of glycyrrhetic acid in rat bile

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Title Glucuronide-sulfate diconjugate as a novel metabolite of glycyrrhetic acid in rat bile
Author Jing, Jing; Ren, Weichao; Chen, Xijing; Wang, Yue; Yu, Qiaoling; Wang, Guangji; Davey, Andrew; Wang, Jiping; Jing, Ning
Journal Name Drug Metabolism and Pharmacokinetics
Year Published 2008
Place of publication Japan
Publisher Yakubutsu Dotai Gakkai
Abstract To study the metabolites of glycyrrhetic acid (GA) in rat bile, an analytical method was developed to identify GA and its metabolites by liquid chromatography mass spectrometry (LC-MS). Rat bile was collected after i.v. injection of GA. Three major GA-related peaks were detectable in rat bile by high-performance liquid chromatography (HPLC) analysis at 254 nm. LC-MS spectra showed their protonated molecular ions at m/z 727, 647, and 551. Furthermore, the three metabolites were also confirmed to exist in rat bile on LC-MS total ion chromatogram (TIC). Taken together with the susceptible nature to β-glucuronidase digestion and alkaline conditions, they were identified to be a novel sulfate-glucuronide diconjugate and the known monoglucuronide and sulfate conjugate, respectively.
Peer Reviewed Yes
Published Yes
Publisher URI
Copyright Statement Copyright 2008 The Japanese Society for the Study of Xenobiotics (JSSX). The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the conference's website for access to the definitive, published version.
Volume 23
Issue Number 3
Page from 175
Page to 180
ISSN 1347-4367
Date Accessioned 2011-06-22
Language en_AU
Research Centre Molecular Basis of Disease
Faculty Griffith Health Faculty
Subject Basic Pharmacology
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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