Differential Expression of 14-3-3 Isoforms in Human Alcoholic Brain

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Title Differential Expression of 14-3-3 Isoforms in Human Alcoholic Brain
Author Okolicsanyi, Rachel; Colson, Natalie Jane; Dodd, Peter R.; Lewohl, Joanne Marie
Journal Name Alcoholism: Clinical and Experimental Research
Year Published 2011
Place of publication United States
Publisher Wiley-Blackwell Publishing, Inc.
Abstract Keywords: Alcohol Abuse; Gene Expression; Ethanol; Real-Time PCR Background:  Neuropathological damage as a result of chronic alcohol abuse often results in the impairment of cognitive function. The damage is particularly marked in the frontal cortex. The 14-3-3 protein family consists of 7 proteins, β, γ, ε, ζ, η, θ, and σ, encoded by 7 distinct genes. They are highly conserved molecular chaperones with roles in the regulation of metabolism, signal transduction, cell-cycle control, protein trafficking, and apoptosis. They may also play an important role in neurodegeneration in chronic alcoholism. Methods:  We used real-time PCR to measure the expression of 14-3-3 mRNA transcripts in both the dorsolateral prefrontal cortex and motor cortex of human brains obtained at autopsy. Results:  We found significantly lower 14-3-3β, γ, and θ expression in both cortical areas of alcoholics, but no difference in 14-3-3η expression, and higher expression of 14-3-3σ in both areas. Levels of 14-3-3ζ and ε transcripts were significantly lower only in alcoholic motor cortex. Conclusions:  Altered 14-3-3 expression could contribute to synaptic dysfunction and altered neurotransmission in chronic alcohol misuse by human subjects
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1111/j.1530-0277.2011.01436.x
Volume 35
Issue Number 6
Page from 1041
Page to 1049
ISSN 0145-6008
Date Accessioned 2011-06-20
Language en_AU
Research Centre Menzies Health Institute Qld; Molecular Basis of Disease
Faculty Griffith Health Faculty
Subject Central Nervous System; Gene Expression (incl Microarray and other genome-wide approaches)
URI http://hdl.handle.net/10072/41196
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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