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dc.contributor.authorYao, Qin
dc.contributor.authorCao, Siyu
dc.contributor.authorLi, Chun
dc.contributor.authorMengesha, Asferd
dc.contributor.authorKong, Beihua
dc.contributor.authorWei, Mingqian
dc.date.accessioned2018-01-25T12:30:49Z
dc.date.available2018-01-25T12:30:49Z
dc.date.issued2011
dc.date.modified2011-11-08T08:02:31Z
dc.identifier.issn0020-7136
dc.identifier.doi10.1002/ijc.25506
dc.identifier.urihttp://hdl.handle.net/10072/41450
dc.description.abstractTransforming growth factor-β1 (TGF-β1) induces stromal fibroblast-to-myofibroblast transdifferentiation in the tumor-stroma interactive microenvironment via modulation of multiple phenotypic and functional genes, which plays a critical role in tumor progression. Up to now, the involvement of micro-RNAs (miRNAs) and their roles in TGF-β1-induced myofibroblast differentiation in tumor-stroma interaction are unclear. Using quantitative real-time RT-PCR, we demonstrated that the expression of micro-RNA-21 (miR-21) was upregulated in activated fibroblasts after treatment with TGF-β1 or conditioned medium from cancer cells. To determine the potential roles of miR-21 in TGF-β1-mediated gene regulation during myofibroblast conversion, we showed that miR-21 expression was downregulated by miR-21 inhibitor and upregulated by miR-21 mimic. Interestingly, downregulation of miR-21 with the inhibitor effectively inhibited TGF-β1-induced myofibroblast differentiation while upregulation of miR-21 with a mimic significantly promoted myofibroblast differentiation. We further demonstrated that MiR-21 directly targeted and downregulated programmed cell death 4 (PDCD4) gene, which in turn acted as a negative regulator of several phenotypic and functional genes of myofibroblasts. Taken together, these results suggested that miR-21 participated in TGF-β1-induced myofibroblast transdifferentiation in cancer stroma by targeting PDCD4.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherJohn Wiley & Sons, Inc.
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1783
dc.relation.ispartofpageto1792
dc.relation.ispartofissue8
dc.relation.ispartofjournalInternational Journal of Cancer
dc.relation.ispartofvolume128
dc.rights.retentionY
dc.subject.fieldofresearchOncology and carcinogenesis
dc.subject.fieldofresearchCancer cell biology
dc.subject.fieldofresearchcode3211
dc.subject.fieldofresearchcode321101
dc.titleMicro-RNA-21 regulates TGF-β-induced myofibroblast differentiation by targeting PDCD4 in tumor-stroma interaction
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionAccepted Manuscript (AM)
gro.facultyGriffith Health, School of Medical Science
gro.rights.copyright© 2011 UICC. This is the pre-peer reviewed version of the following article: MicroRNA-21 regulates TGF-beta-induced myofibroblast differentiation by targeting PDCD4 in tumour-stroma interaction, International Journal of Cancer, Vol128 (8), 2011, pp.1783-1792, which has been published in final form at http://dx.doi.org/10.1002/ijc.25506. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
gro.date.issued2011
gro.hasfulltextFull Text
gro.griffith.authorWei, Ming Q.


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