dc.contributor.author | Yao, Qin | |
dc.contributor.author | Cao, Siyu | |
dc.contributor.author | Li, Chun | |
dc.contributor.author | Mengesha, Asferd | |
dc.contributor.author | Kong, Beihua | |
dc.contributor.author | Wei, Mingqian | |
dc.date.accessioned | 2018-01-25T12:30:49Z | |
dc.date.available | 2018-01-25T12:30:49Z | |
dc.date.issued | 2011 | |
dc.date.modified | 2011-11-08T08:02:31Z | |
dc.identifier.issn | 0020-7136 | |
dc.identifier.doi | 10.1002/ijc.25506 | |
dc.identifier.uri | http://hdl.handle.net/10072/41450 | |
dc.description.abstract | Transforming growth factor-β1 (TGF-β1) induces stromal fibroblast-to-myofibroblast transdifferentiation in the tumor-stroma interactive microenvironment via modulation of multiple phenotypic and functional genes, which plays a critical role in tumor progression. Up to now, the involvement of micro-RNAs (miRNAs) and their roles in TGF-β1-induced myofibroblast differentiation in tumor-stroma interaction are unclear. Using quantitative real-time RT-PCR, we demonstrated that the expression of micro-RNA-21 (miR-21) was upregulated in activated fibroblasts after treatment with TGF-β1 or conditioned medium from cancer cells. To determine the potential roles of miR-21 in TGF-β1-mediated gene regulation during myofibroblast conversion, we showed that miR-21 expression was downregulated by miR-21 inhibitor and upregulated by miR-21 mimic. Interestingly, downregulation of miR-21 with the inhibitor effectively inhibited TGF-β1-induced myofibroblast differentiation while upregulation of miR-21 with a mimic significantly promoted myofibroblast differentiation. We further demonstrated that MiR-21 directly targeted and downregulated programmed cell death 4 (PDCD4) gene, which in turn acted as a negative regulator of several phenotypic and functional genes of myofibroblasts. Taken together, these results suggested that miR-21 participated in TGF-β1-induced myofibroblast transdifferentiation in cancer stroma by targeting PDCD4. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | John Wiley & Sons, Inc. | |
dc.publisher.place | United States | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 1783 | |
dc.relation.ispartofpageto | 1792 | |
dc.relation.ispartofissue | 8 | |
dc.relation.ispartofjournal | International Journal of Cancer | |
dc.relation.ispartofvolume | 128 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Oncology and carcinogenesis | |
dc.subject.fieldofresearch | Cancer cell biology | |
dc.subject.fieldofresearchcode | 3211 | |
dc.subject.fieldofresearchcode | 321101 | |
dc.title | Micro-RNA-21 regulates TGF-β-induced myofibroblast differentiation by targeting PDCD4 in tumor-stroma interaction | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
dc.description.version | Accepted Manuscript (AM) | |
gro.faculty | Griffith Health, School of Medical Science | |
gro.rights.copyright | © 2011 UICC. This is the pre-peer reviewed version of the following article: MicroRNA-21 regulates TGF-beta-induced myofibroblast differentiation by targeting PDCD4 in tumour-stroma interaction, International Journal of Cancer, Vol128 (8), 2011, pp.1783-1792, which has been published in final form at http://dx.doi.org/10.1002/ijc.25506. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html) | |
gro.date.issued | 2011 | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Wei, Ming Q. | |