An investigation of the activity of recomibant rat skeletal muscle cytosolic sialidase

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Title An investigation of the activity of recomibant rat skeletal muscle cytosolic sialidase
Author Albouz-Abo, Samia; Turton, Richard; Wilson, Jenny; von Itzstein, Mark
Journal Name FEBS Letters
Editor Gerrit van Meer
Year Published 2005
Place of publication Amsterdam, The Netherlands
Publisher Elsevier B.V.
Abstract Telomerase activation is regulated by the expression of human telomerase reverse transcriptase (hTERT) and is a key step in the development of human cancers. Interferon-γ (IFN-γ) signaling induces growth arrest in many tumors through multiple regulatory mechanisms. The p27 tumor suppressor protein inhibits the formation of tumors through the induction of cell cycle arrest and/or apoptosis. We demonstrate here that p27Kip1 inhibits hTERT mRNA expression and telomerase activity through post-transcriptional up-regulation by IFN-γ/IRF-1 signaling. The ectopic expression of p27 suppressed hTERT expression and telomerase activity in human cervical cancer cell lines, HeLa and HT3. Furthermore, hTERT promoter activity of mouse embryonic fibroblasts (MEFs) deficient in p27 (p27−/− MEFs) was significantly higher than that of wild-type MEFs. Overexpression of p27 suppressed hTERT promoter activity and telomerase activity of p27−/− MEFs. In addition p27 down-regulated E7 protein expression and in transiently transfected HeLa cells, E7 increased hTERT promoter activity. In conclusion, we propose that inhibition of the hTERT expression and telomerase activity may be a novel tumor suppressor function of p27.
Peer Reviewed Yes
Published Yes
Publisher URI http://www.elsevier.com/wps/find/journaldescription.cws_home/506085/description#description
Alternative URI http://dx.doi.org/10.1016/j.febslet.2004.12.077
Copyright Statement Copyright 2005 Elsevier. Please refer to the journal's website for access to the definitive, published version.
Volume 579
Issue Number 5
Page from 1034
Page to 1038
ISSN 0014-5793
Date Accessioned 2006-03-09
Date Available 2012-02-10T01:43:23Z
Language en_US
Research Centre Institute for Glycomics
Faculty Institute for Glycomics
Subject PRE2009-Enzymes
URI http://hdl.handle.net/10072/4186
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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