Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the VP8* carbohydrate-binding protein of the human rotavirus strain Wa.

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Title Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the VP8* carbohydrate-binding protein of the human rotavirus strain Wa.
Author Kraschnefski, Mark; Scott, Stacy; Holloway, Gavan; Coulson, Barbara S.; von Itzstein, Mark; Blanchard, Helen
Journal Name Acta Crystallographica Section F - Structural Biology and Crystallization Communications
Editor H. M. Einspahr and J. M. Guss
Year Published 2005
Place of publication Oxford, England
Publisher Blackwell Publishing
Abstract Rotaviruses exhibit host-specificity and the first crystallographic information on a rotavirus strain that infects humans is reported here. Recognition and attachment to host cells, leading to invasion and infection, is critically linked to the function of the outer capsid spike protein of the rotavirus particle. In some strains the VP8* component of the spike protein is implicated in recognition and binding of sialic-acid-containing cell-surface carbohydrates, thereby enabling infection by the virus. The cloning, expression, purification, crystallization and initial X-ray diffraction analysis of the VP8* core from human Wa rotavirus is reported. Two crystal forms (trigonal P3221 and monoclinic P21) have been obtained and X-ray diffraction data have been collected, enabling determination of the VP8*64-223 structure by molecular replacement.
Peer Reviewed Yes
Published Yes
Publisher URI http://www.blackwell-synergy.com/doi/full/10.1107/S1744309105032999
Alternative URI http://dx.doi.org/10.1107/S1744309105032999
Copyright Statement Copyright 2005 Blackwell Publishing. The definitive version is available at [www.blackwell-synergy.com.]
Volume F61
Page from 989
Page to 993
ISSN 1744-3091
Date Accessioned 2006-03-09
Language en_AU
Research Centre Institute for Glycomics
Faculty Institute for Glycomics
Subject PRE2009-Biological and Medical Chemistry
URI http://hdl.handle.net/10072/4189
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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