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dc.contributor.authorKraschnefski, MJ
dc.contributor.authorScott, SA
dc.contributor.authorHolloway, G
dc.contributor.authorCoulson, BS
dc.contributor.authorvon Itzstein, M
dc.contributor.authorBlanchard, H
dc.contributor.editorH. M. Einspahr and J. M. Guss
dc.date.accessioned2018-01-23T01:00:21Z
dc.date.available2018-01-23T01:00:21Z
dc.date.issued2005
dc.identifier.issn1744-3091
dc.identifier.doi10.1107/S1744309105032999
dc.identifier.urihttp://hdl.handle.net/10072/4189
dc.description.abstractRotaviruses exhibit host-specificity and the first crystallographic information on a rotavirus strain that infects humans is reported here. Recognition and attachment to host cells, leading to invasion and infection, is critically linked to the function of the outer capsid spike protein of the rotavirus particle. In some strains the VP8* component of the spike protein is implicated in recognition and binding of sialic-acid-containing cell-surface carbohydrates, thereby enabling infection by the virus. The cloning, expression, purification, crystallization and initial X-ray diffraction analysis of the VP8* core from human Wa rotavirus is reported. Two crystal forms (trigonal P3221 and monoclinic P21) have been obtained and X-ray diffraction data have been collected, enabling determination of the VP8*64-223 structure by molecular replacement.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.publisher.placeOxford, England
dc.relation.ispartofstudentpublicationY
dc.relation.ispartofpagefrom989
dc.relation.ispartofpageto993
dc.relation.ispartofjournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
dc.relation.ispartofvolumeF61
dc.rights.retentionY
dc.subject.fieldofresearchChemical sciences
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchcode34
dc.subject.fieldofresearchcode31
dc.titleCloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the VP8* carbohydrate-binding protein of the human rotavirus strain Wa.
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2015-01-30T00:43:16Z
gro.hasfulltextNo Full Text
gro.griffith.authorvon Itzstein, Mark


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