New personal sampler for viable airborne viruses: feasibility study

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Title New personal sampler for viable airborne viruses: feasibility study
Author Agranovski, Igor E; Safatov, A.S.; Borodulin, A.I.; Pyankov, O.V.; Petrishchenko, V.A.; Sergeev, A.N.; Sergeev, A.A.; Agranovski, V.; Grinshpun, S.A.
Year Published 2005
Place of publication United Kingdom
Publisher PERGAMON
Abstract While various sampling methods exist for collecting and enumerating airborne bacteria and fungi, no credible methodology has yet been developed for airborne viruses. A new sampling method for monitoring the personal exposure to bioaerosol particles has recently been developed and evaluated with bacteria and fungi. In this method, bacterial/fungal aerosol is aspirated and transported through a porous medium, which is submerged into a liquid layer. As the air is split into numerous bubbles, the particles are scavenged by these bubbles and effectively removed. The current feasibility study was initiated to evaluate the efficiency of the new personal sampler prototype (bubbler) with airborne viable viruses. Two common viral strains, Influenza (stress-sensitive) and Vaccinia (robust), were aerosolized in the test chamber and collected by two identical bubblers that operated simultaneously for a duration of upto 5 min. A virus maintenance liquid, proven to be the optimum collecting environment for the test organisms, was used as a collection fluid. After sampling, the collecting fluid was analyzed and the viral recovery rate was determined. The overall recovery (affected not only by the sampling but also by the aerosolization and the aerosol transport) was 20% for Influenza virus and 89% for Vaccinia virus. The new sampling method was found feasible for the collection and enumeration of robust airborne viruses.
Peer Reviewed Yes
Published Yes
Publisher URI
Copyright Statement Copyright 2005 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links.
Volume 36
Page from 609
Page to 617
ISSN 0021-8502
Date Accessioned 2006-03-01
Language en_AU
Faculty Faculty of Engineering and Information Technology
Subject Environmental Technologies
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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