Show simple item record

dc.contributor.authorZalucki, Yaramah M
dc.contributor.authorShafer, William M
dc.contributor.authorJennings, Michael P
dc.date.accessioned2018-02-09T12:30:28Z
dc.date.available2018-02-09T12:30:28Z
dc.date.issued2011
dc.date.modified2012-03-07T05:38:20Z
dc.identifier.issn0005-2736
dc.identifier.doi10.1016/j.bbamem.2011.06.004
dc.identifier.urihttp://hdl.handle.net/10072/43392
dc.description.abstractSignal sequence non-optimal codons have been shown to be important for the folding and efficient export of maltose binding protein (MBP), a SecB dependent protein. In this study, we analysed the importance of signal sequence non-optimal codons of TolB, a signal recognition particle (SRP) dependent exported protein. The protein production levels of wild type TolB (TolB-wt) and a mutant allele of TolB in which all signal sequence non-optimal codons were changed to a synonymous optimal codon (TolB-opt), revealed that TolB-opt production was 12-fold lower than TolB-wt. This difference could not be explained by changes in mRNA levels, or plasmid copy number, which was the same in both strains. A directed evolution genetic screen was used to select for mutants in the TolB-opt signal sequence that resulted in higher levels of TolB production. Analysis of the 46 independent TolB mutants that reverted to wild type levels of expression revealed that at least four signal sequence non-optimal codons were required. These results suggest that non-optimal codons may be required for the folding and efficient export of all proteins exported via the Sec system, regardless of whether they are dependent on SecB or SRP for delivery to the inner membrane.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom2544
dc.relation.ispartofpageto2550
dc.relation.ispartofissue10
dc.relation.ispartofjournalBiochimica et Biophysica Acta-Biomembranes
dc.relation.ispartofvolume1808
dc.rights.retentionY
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchOther biological sciences
dc.subject.fieldofresearchChemical engineering
dc.subject.fieldofresearchMedical microbiology not elsewhere classified
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3199
dc.subject.fieldofresearchcode4004
dc.subject.fieldofresearchcode320799
dc.titleDirected evolution of efficient secretion in the SRP-dependent export of TolB
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2011
gro.hasfulltextNo Full Text
gro.griffith.authorJennings, Michael P.
gro.griffith.authorZalucki, Yaramah


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record