The Targeted Expression of Nucleotide Sugar Transporters to the E. coli Inner Membrane

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Title The Targeted Expression of Nucleotide Sugar Transporters to the E. coli Inner Membrane
Author Tiralongo, Joe; Maggioni, Andrea
Journal Name Methods in Molecular Biology
Year Published 2011
Place of publication United States
Publisher Springer
Abstract The heterologous expression of functional mammalian integral membrane proteins still represents a significant hurdle towards evaluating the relationship between their structure and function. We have therefore utilised the OmpA signal sequence to deliberately target the expression of a mammalian nucleotide sugar transporter, the murine CMP-sialic acid transporter, to the E. coli inner membrane. The functionality of the recombinant CMP-sialic acid transporter could then be evaluated either following the spheroplasting of E. coli cells or through the isolation of the E. coli inner membrane and the formation of mixed phosphatidylcholine-inner membrane proteoliposomes.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1007/978-1-61737-967-3_14
Copyright Statement Copyright 2011 Springer. This is an electronic version of an article published in Methods in Molecular Biology, Vol. 705, 2011, pp. 237-249. Methods in Molecular Biology is available online at: http://link.springer.com// with the open URL of your article.
Volume 705
Page from 237
Page to 249
ISSN 1064-3745
Date Accessioned 2012-02-17
Language en_US
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Receptors and Membrane Biology
URI http://hdl.handle.net/10072/43425
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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