Differential Carbohydrate Binding and Cell Surface Glycosylation of Human Cancer Cell Lines

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Title Differential Carbohydrate Binding and Cell Surface Glycosylation of Human Cancer Cell Lines
Author Arndt, Nadia; Tiralongo, Joe; Madge, Paul David; von Itzstein, Mark; Day, Christopher
Journal Name Journal of Cellular Biochemistry
Year Published 2011
Place of publication United States
Publisher John Wiley & Sons inc.
Abstract Currently there is only a modest level knowledge of the glycosylation status of immortalised cell lines that are commonly used in cancer biology as well as their binding affinities to different glycan structures. Through use of glycan and lectin microarray technology, this study has endeavoured to define the different bindings of cell surface carbohydrate structures to glycan-binding lectins. The screening of breast cancer MDA-MB435 cells, cervical cancer HeLa cells and colon cancer Caco-2, HCT116 and HCT116–FM6 cells was conducted to determine their differential bindings to a variety of glycan and lectin structures printed on the array slides. An inverse relationship between the number of glycan structures recognised and the variety of cell surface glycosylation was observed. Of the cell lines tested, it was found that four bound to sialylated structures in initial screening. Secondary screening in the presence of a neuraminidase inhibitor (4-deoxy-4-guanidino- Neu5Ac2en) significantly reduced sialic acid binding. The array technology has proven to be useful in determining the glycosylation signatures of various cell-lines as well as their glycan binding preferences. The findings of this study provide the groundwork for further investigation into the numerous glycan–lectin interactions that are exhibited by immortalised cell lines.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1002/jcb.23139
Volume 112
Issue Number 9
Page from 2230
Page to 2240
ISSN 0730-2312
Date Accessioned 2012-02-20; 2012-03-08T22:32:45Z
Date Available 2012-03-08T22:32:45Z
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Cancer Cell Biology; Cellular Interactions (incl Adhesion, Matrix, Cell Wall); Receptors and Membrane Biology
URI http://hdl.handle.net/10072/43447
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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