A novel nickel responsive MerR-like regulator, NimR, from Haemophilus influenzae

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Title A novel nickel responsive MerR-like regulator, NimR, from Haemophilus influenzae
Author Kidd, Stephen P.; Djoko, Karrera Y.; Ng, JiaQi; Argente, M. Pilar; Jennings, Michael Paul; McEwan, Alastair G.
Journal Name Metallomics: integrated biometal science
Year Published 2011
Place of publication United Kingdom
Publisher Royal Society of Chemistry
Abstract We have identified a novel regulator from the MerR family of transcription factors in the bacterial pathogen Haemophilus influenzae (HI1623; nickel-associated merR-like Regulator – NimR). NimR regulates the expression of a Ni2+ uptake transporter (NikKLMQO). The promoters for nimR and the nik operon are divergent and overlapping and NimR binds at a site between the promoter elements for nikKLMQO. Expression of this operon requires NimR and depends on Ni2+. Growth rates of the H. influenzae nimR and nikQ mutants were reduced in chemically defined media compared to the wild type and the mutants were unable to grow in the presence of EDTA. The mutant strains were less tolerant of acidic pH and the wild type Rd KW20 could not tolerate low pH in the presence of fluoramide, a urease specific inhibitor, confirming that both nickel transport and urea hydrolysis are a central process in pH control. H. influenzae nimR and nikQ strains were deficient in urease activity, but this could be specifically restored by the addition of excess Ni2+. NimR did not directly regulate the expression of urease genes but the activity of urease requires both nimR and nikQ. Purified NimR is a dimer that binds 1 Ni2+ion. NimR is the first example of a Ni-dependent regulator from the MerR family and targeting a metal ion uptake system; it is distinct from NikR the Ni-responsive regulators of the ribbon-helix-helix family.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1039/C1MT00127B
Volume 3
Issue Number 10
Page from 1009
Page to 1018
ISSN 1756-5901
Date Accessioned 2012-03-05; 2012-04-01T22:39:43Z
Date Available 2012-04-01T22:39:43Z
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Medical Microbiology
URI http://hdl.handle.net/10072/44113
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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