Show simple item record

dc.contributor.authorKidd, Stephen P
dc.contributor.authorDjoko, Karrera Y
dc.contributor.authorNg, JiaQi
dc.contributor.authorArgente, M Pilar
dc.contributor.authorJennings, Michael P
dc.contributor.authorMcEwan, Alastair G
dc.date.accessioned2017-05-03T13:12:18Z
dc.date.available2017-05-03T13:12:18Z
dc.date.issued2011
dc.date.modified2012-04-01T22:39:43Z
dc.identifier.issn1756-5901
dc.identifier.doi10.1039/C1MT00127B
dc.identifier.urihttp://hdl.handle.net/10072/44113
dc.description.abstractWe have identified a novel regulator from the MerR family of transcription factors in the bacterial pathogen Haemophilus influenzae (HI1623; nickel-associated merR-like Regulator - NimR). NimR regulates the expression of a Ni2+ uptake transporter (NikKLMQO). The promoters for nimR and the nik operon are divergent and overlapping and NimR binds at a site between the promoter elements for nikKLMQO. Expression of this operon requires NimR and depends on Ni2+. Growth rates of the H. influenzae nimR and nikQ mutants were reduced in chemically defined media compared to the wild type and the mutants were unable to grow in the presence of EDTA. The mutant strains were less tolerant of acidic pH and the wild type Rd KW20 could not tolerate low pH in the presence of fluoramide, a urease specific inhibitor, confirming that both nickel transport and urea hydrolysis are a central process in pH control. H. influenzae nimR and nikQ strains were deficient in urease activity, but this could be specifically restored by the addition of excess Ni2+. NimR did not directly regulate the expression of urease genes but the activity of urease requires both nimR and nikQ. Purified NimR is a dimer that binds 1 Ni2+ion. NimR is the first example of a Ni-dependent regulator from the MerR family and targeting a metal ion uptake system; it is distinct from NikR the Ni-responsive regulators of the ribbon-helix-helix family.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherRoyal Society of Chemistry
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1009
dc.relation.ispartofpageto1018
dc.relation.ispartofissue10
dc.relation.ispartofjournalMetallomics: integrated biometal science
dc.relation.ispartofvolume3
dc.rights.retentionY
dc.subject.fieldofresearchChemical sciences
dc.subject.fieldofresearchMedical microbiology not elsewhere classified
dc.subject.fieldofresearchcode34
dc.subject.fieldofresearchcode320799
dc.titleA novel nickel responsive MerR-like regulator, NimR, from Haemophilus influenzae
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2011
gro.hasfulltextNo Full Text
gro.griffith.authorJennings, Michael P.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record