Transcriptional profiling of "guided bone regeneration" in a critical-size calvarial defect

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Title Transcriptional profiling of "guided bone regeneration" in a critical-size calvarial defect
Author Ivanovski, Saso; Hamlet, Stephen; Retzepi, M.; Wall, I.; Donos, N
Journal Name Clinical Oral Implants Research
Year Published 2011
Place of publication United States
Publisher Wiley-Blackwell Publishing, Inc.
Abstract Objectives: Guided bone regeneration (GBR) is a commonly utilized surgical technique in the craniofacial region. The transcriptional mechanisms associated with this type of bone regeneration are not well understood. The aim of this study was to characterize the transcriptome associated with GBR of a critical-size calvarial defect in the rat. Material and methods: Critical-size calvarial defects were created in six Wistar strain rats and treated according to the principles of GBR. The tissue filling the regenerating defect was harvested at 7 and 14 days. Total RNA was extracted and microarray analysis was carried out to identify the differences in the transcriptome between days 7 and 14. Results: Gene ontology (GO) analysis of the genes up-regulated at day 7 showed that immature wound healing-related mechanisms, such as protein metabolism and cell proliferation, were upregulated at this time point. Furthermore, the immuno-inflammatory process was also up-regulated at the earlier time point. In contrast, by day 14, GO groups consistent with wound maturation, such as extracellular matrix formation, anatomical structure development and cell differentiation, were upregulated. Furthermore, the functionally important GO categories of skeletal development, ossification and bone mineralization were up-regulated at day 14. Genes of interest that belonged to this group and were up-regulated at day 14 included growth and differentiation factors (Bmp2, Bmp3, Tgfb3), extracellular matrix proteins (osteocalcin, osteomodulin, stenniocalcin 1) and transcription factors (Runx2, Sox6, Satb2). Furthermore, a number of genes associated with Tgfb/Bmp and Wnt signalling were also up-regulated. Besides skeletogenesis, genes associated with angiogenesis and neurogenesis were also up-regulated at day 14. Conclusions: The transcriptome associated with a maturing GBR-treated craniofacial bone defect is characterized by the down-regulation of the immuno-inflammatory response and up-regulation of skeletogeneis-, angiogenesis- and neurogenesis-associated genes. The Tgfb/Bmp and Wnt signalling pathways play an important role in the regenerative process.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1111/j.1600-0501.2010.02104.x
Volume 22
Issue Number 4
Page from 382
Page to 389
ISSN 0905-7161
Date Accessioned 2012-03-14; 2012-04-09T22:56:31Z
Date Available 2012-04-09T22:56:31Z
Research Centre Griffith Health Institute; Molecular Basis of Disease
Faculty Griffith Health Faculty
Subject Biomedical Engineering
URI http://hdl.handle.net/10072/44289
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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