Lectin Array Analysis of Purified Lipooligosaccharide: A Method for the Determination of Molecular Mimicry

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Title Lectin Array Analysis of Purified Lipooligosaccharide: A Method for the Determination of Molecular Mimicry
Author Semchenko, Evgeny; Moutin, Marc; Korolik, Victoria; Tiralongo, Joe; Day, Christopher
Journal Name Journal of Glycomics and Lipidomics
Year Published 2011
Place of publication United States
Publisher OMICS Publishing Group
Abstract Surface glycosylation of bacteria is involved in many critical host-microbe interactions. Lectin arrays consisting of diverse carbohydrate binding proteins have proven to be an important tool for evaluating a wide variety of glycosylation, including that present on whole bacteria. However, assessing glycosylation on whole bacteria using lectin arrays may not reflect bacterial glycosylation, but interactions between bacteria and the glycosylation present on lectins. The lipooligosaccharide of Campylobacter jejuni NCTC11168 and 81-176 are known to mimic the human monosialylated gangliosides. This molecular mimicry by C. jejuni can result in the post infection sequelae Guillain-Barré syndrome. Using C. jejuni as a model system and a discreet lectin and antibody array, a method, applicable to many organisms has been developed and validated by to screening of the purified lipooligosaccharide of C. jejuni for molecular mimicry to monosialylated gangliodises. In case of C. jejuni, knowing whether clinically important bacterial strains are capable of inducing severe autoimmune responses may aid in prevention and/or early diagnosis of debilitating post infection conditions.
Peer Reviewed Yes
Published Yes
Publisher URI http://www.omicsonline.org/jglhome.php
Copyright Statement Copyright remains with the authors 2011. The attached file is reproduced here in accordance with the copyright policy of the publisher. For information about this journal please refer to the journal’s website or contact the authors.
Volume 1
Issue Number 3
Page from 1000105-1
Page to 1000105-5
ISSN 2153-0637
Date Accessioned 2012-02-22; 2012-05-09T22:52:52Z
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Microbiology
URI http://hdl.handle.net/10072/44993
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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