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dc.contributor.authorMotherby, Helma
dc.contributor.authorNadjari, Bahram
dc.contributor.authorRemmerbach, Torsten Wilhelm
dc.contributor.authorMarcy, Tobias
dc.contributor.authorPomjanskaja, Natalia
dc.contributor.authorMüller, Wolfram
dc.contributor.authorKnops, Kristiane
dc.contributor.authorHäussinger, Dieter
dc.contributor.authorStrauer, Bodo-Eckehard
dc.contributor.authorBӧcking, Alfred
dc.date.accessioned2007-06-27
dc.date.accessioned2017-03-01T23:05:31Z
dc.date.available2015-06-01T23:37:23Z
dc.date.available2017-03-01T23:05:31Z
dc.date.issued1998
dc.identifier.issn0884-6812
dc.identifier.urihttp://hdl.handle.net/10072/45040
dc.description.abstractOBJECTIVE: The sensitivity of conventional cytology for identification of neoplastic cells in effusions is unsatisfactory, about 58%. The rate of diagnostically equivocal effusions in routine cytology is about 6%. DNA aneuploidy has previously been proven to be a sensitive and specific marker for the identification of tumor cells in effusions. In the present study we determined if malignancy can be identified in cytologically equivocal cells in effusions using DNA aneuploidy as a marker, thus decreasing the rate of cytologically equivocal diagnoses in effusions. STUDY DESIGN: One hundred cytologically equivocal effusions of the serous cavities were obtained from routine diagnostic material. Nuclear DNA content was measured after Feulgen staining using a TV image analysis system. Data were correlated with patient follow-up. RESULTS: DNA aneuploidy was assumed if abnormal DNA stemlines, a coefficient of variation of the first DNA stemline > or = 10% or cells > 9c were observed. The sensitivity of DNA aneuploidy for the identification of malignancy was 55.9%. Specificity of DNA nonaneuploidy for benignity was 94.1%. The positive predictive value of the marker DNA aneuploidy for the occurrence of malignant cells was 97.9% since all but one DNA aneuploid case showed malignancy in follow-up. CONCLUSION: Image cytometry applying DNA aneuploidy as a parameter is able to detect the occurrence of malignant cells in cytologically equivocal effusions in about every second case. Thus, this method is able to increase diagnostic accuracy of conventional effusion cytology by decreasing the rate of diagnostically equivocal effusions.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherScience Printers
dc.publisher.placeUnited States
dc.publisher.urihttp://www.aqch.com/toc/auto_abstract.php?id=9535
dc.relation.ispartofpagefrom162
dc.relation.ispartofpageto168
dc.relation.ispartofissue3
dc.relation.ispartofjournalAnal Quant Cytol Histol
dc.relation.ispartofvolume20
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchOncology and Carcinogenesis
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1112
dc.titleStatic DNA cytometry as a diagnostic aid in effusion cytology: II. DNA aneuploidy for identification of neoplastic cells in equivocal effusions
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codec1x
gro.facultyGriffith Health Faculty
gro.date.issued2015-06-01T23:37:23Z
gro.hasfulltextNo Full Text
gro.griffith.authorRemmerbach, Torsten W.


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