Genetic variation of IL-12B (+1188 region) is associated with its decreased circulating levels and susceptibility to Type 2 diabetes

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Title Genetic variation of IL-12B (+1188 region) is associated with its decreased circulating levels and susceptibility to Type 2 diabetes
Author Yaghini, Narges; Mahmoodi, Mehdi; Hassanshahi, Gholamhossein; Asadikaram, Gholamreza; Arababadi, Mohammad Kazemi; Rezaeian, Mohsen; Sajjadi, Seyed Mohammad Ali; Kennedy, Derek
Journal Name Biomarkers in Medicine
Year Published 2012
Place of publication United Kingdom
Publisher Future Medicine Ltd.
Abstract Background: Type 2 diabetes mellitus is one of the most common types of endocrine disease and the immune system plays a predominant role in its pathogenesis. Aims: The present study aimed to examine known gene polymorphisms within IL-12B (+1188) region and its circulating serum levels in Type 2 diabetic patients from the southeastern region of Iran and compare them with unrelated controls. Materials & methods: In this clinical study, peripheral blood was collected from 114 Type 2 diabetic patients and 100 healthy controls. Serum levels of IL-12B were measured by ELISA. Genomic DNA was extracted from peripheral blood samples and polymorphisms at the +1188 position of the IL-12B gene were assessed using PCR restriction fragment-length polymorphism. Results: Our findings demonstrated that the AA genotype and the A allele of IL-12B were increased significantly in Type 2 diabetic patients when compared with controls. Our results also showed that the circulating levels of IL-12B were significantly decreased in Type 2 diabetic patients when compared with controls. Conclusion: According to the findings of the current study, we concluded that IL-12B and its +1188 polymorphism may play a prominent role in the pathogenesis of Type 2 diabetes. Further replicative investigations using a larger sample size are essential to identify additional IL-12B genetic variants associated with a risk of Type 2 diabetes.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.2217/BMM.11.84
Copyright Statement Copyright 2012 Future Medicine Ltd. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
Volume 6
Issue Number 1
Page from 89
Page to 95
ISSN 1752-0363
Date Accessioned 2012-02-21
Language en_US
Research Centre Eskitis Institute for Drug Discovery
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Medical Genetics (excl Cancer Genetics); Nephrology and Urology
URI http://hdl.handle.net/10072/45633
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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