Optical bioluminescence imaging of human ES cell progeny in the rodent CNS

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Title Optical bioluminescence imaging of human ES cell progeny in the rodent CNS
Author Bradbury, Michelle S.; Panagiotakos, Georgia; Chan, Bill K.; Tomishima, Mark; Zanzonico, Pat; Vider, Jelena; Ponomarev, Vladimir; Studer, Lorenz; Tabar, Viviane
Journal Name Journal of Neurochemistry
Year Published 2007
Place of publication United Kingdom
Publisher Wiley-Blackwell Publishing Ltd.
Abstract Pre-clinical efforts of grafting human embryonic stem cell (hESC)-derived neural precursors have been hampered by problems ranging from graft rejection to overgrowth and tumor formation. The ability to detect such potential complications sensitively and reliably in clinically relevant contexts will rest upon the implementation of suitable non-invasive imaging technologies for continuously probing graft survival, proliferation, and migration. Neural precursors were transduced ex vivo using a lentiviral-mediated gene delivery system expressing firefly D-luciferase, under the control of a cytomegalovirus promoter. Transduced cells revealed no loss of cellular morphology, proliferative capacity, or neural phenotype in vitro. As a novel approach to monitoring the fate of human grafts within the living brain, we adapted optical bioluminescence imaging to assess long-term graft viability in immunodeficient mouse models transplanted with genetically engineered human neural precursor cells. We additionally applied this technology to immunocompetent models for detecting and characterizing the time course of graft rejection. Using this strategy, we define statistically relevant imaging criteria that can predict graft rejection or overgrowth. In conclusion, our data suggest that optical bioluminescence imaging can serve as an essential tool for the development of hESC-based grafting strategies in the CNS.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1111/j.1471-4159.2007.04681.x
Volume 102
Issue Number 6
Page from 2029
Page to 2039
ISSN 0022-3042
Date Accessioned 2012-04-11; 2012-07-12T23:34:49Z
Research Centre Heart Foundation Research Centre; Molecular Basis of Disease
Faculty Griffith Health Faculty
Subject Neurosciences
URI http://hdl.handle.net/10072/45842
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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