Loss of adenosine A2B receptor mediated relaxant responses in the aged female rat bladder; effects of dietary phytoestrogens

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Title Loss of adenosine A2B receptor mediated relaxant responses in the aged female rat bladder; effects of dietary phytoestrogens
Author Owen, Suzzanne; Massa, Helen Maureen; Rose'Meyer, Roselyn Barbara
Journal Name Naunyn-Schmiedeberg Archives of Pharmacology
Editor M.C. Michael
Year Published 2012
Place of publication Germany
Publisher Springer
Abstract This study examined the effect of age, ovariectomy and dietary phytoestrogen ingestion on adenosine A2B receptor mediated relaxant responses and mRNA expression of adenosine receptor subtypes in the rat isolated bladder. Female Wistar rats (8 weeks) were anaesthetised and the ovaries were removed (ovx) or left intact (sham). Rats were fed either normal rat chow (soy, phytoestrogens) or a non-soy (phytoestrogen free) diet. Isolated bladder from rats aged 12, 24 or 52 weeks were pre-contracted with 3 μM carbachol prior to a concentration response curve to 5′-(N-ethylcarboxamido) adenosine (NECA) being obtained. In 12-week-old rats, the bladder exhibited enhanced relaxant responses to NECA in soy-fed rats (P<0.05), whilst at 24 weeks of age, the relaxant responses to NECA were attenuated in all the groups studied except soy-treated sham rat bladders in which the relaxant responses were enhanced. At 52 weeks of age, no relaxant effects were observed in any of the treatment groups and NECA-induced contractile responses occurred. In all bladders, the adenosine A2B receptor was the most abundantly expressed. In bladders from young and mature female rats, the mRNA expression of adenosine receptors (A1, A2A and A2B) was lowest in the bladder from non-soy-fed ovariectomised animals and the use of phytoestrogens in the diet increased the mRNA expression of these receptors (P<0.05).While a soy diet improves the relaxant effects to the adenosine analogue via adenosine A2B receptors in bladders from younger rats, the benefits are lost with advancing age.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1007/s00210-011-0722-y
Copyright Statement Copyright 2012 Springer-Verlag. This is an electronic version of an article published in Naunyn-Schmiedeberg's Archives of Pharmacology, May 2012, Volume 385, Issue 5, pp 539-549. Naunyn-Schmiedeberg's Archives of Pharmacology is available online at: http://link.springer.com// with the open URL of your article.
Volume 385
Issue Number 5
Page from 539
Page to 549
ISSN 0028-1298
Date Accessioned 2012-05-26
Language en_US
Research Centre Griffith Health Institute; Heart Foundation Research Centre
Faculty Griffith Health Faculty
Subject Basic Pharmacology
URI http://hdl.handle.net/10072/46078
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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