T Helper and B Cell Escape Mutations within the HBc Gene in Patients with Asymptomatic HBV Infection: A Study From the South-Eastern Region of Iran

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Title T Helper and B Cell Escape Mutations within the HBc Gene in Patients with Asymptomatic HBV Infection: A Study From the South-Eastern Region of Iran
Author Assar, Shokrollah; Arababadi, Mohammad Kazemi; Mohit, Maryam; Ahmadabadi, Behzad Nasiri; Pumpens, Paul; Khorramdelazad, Hossein; Hajghani, Masomeh; Assar, Sepideh; Araste, Majid; Nekhei, Zohre; Sendi, Hossein; Kennedy, Derek
Journal Name Clinical Laboratory
Year Published 2012
Place of publication Germany
Publisher Clinical Laboratory Publications
Abstract Background: Escape mutations potentially allow viruses to avoid detection and clearance by the host immune sys-tem and may represent a mechanism through which infections may persist in some patients. The association of the mutations in the HBcAg gene with Hepatitis B asymptomatic carriers (ASC) has not been studied adequately. The current study was aimed to investigate HBcAg18-27 CTL epitope mutations in ASC patients in the South-Eastern region of Iran. Methods: 100 ASC patients were selected for this study and screened for HLA-A2 using flow cytometry. HBV-DNA was extracted from the HLA-A2 positive patients and the HBc gene was amplified using PCR. Direct double sequencing was performed to analyse mutations in the HBc gene of HBV isolates from patients with ASC. Results: Overall, 25 (25%) of individuals were HLA-A2 positive. Direct double sequencing indicated no mutations in the HBcAg18-27 epitope. However, four mutations within the T helper and three mutations within the B cell epitopes of ASC patients were identified. Conclusions: The lack of mutations within the HBcAg18-27 epitope suggests that the antigenicity of this region is not altered in HBV isolates of our patients and therefore antigen presentation would occur normally to the pa-tient’s immune system through HLA-A2. However, in the course of this study we revealed some novel mutations within the T helper and B cell epitopes that may affect the efficiencies of immune response of ASC patients against these novel HBV epitopes. (Clin. Lab. 2012;58:53-60)
Peer Reviewed Yes
Published Yes
Publisher URI http://www.clin-lab-publications.com/archive/2012-01-01.html
Volume 58
Page from 53
Page to 60
ISSN 1433-6510
Date Accessioned 2012-02-10
Date Available 2013-06-05T02:07:50Z
Language en_US
Research Centre Eskitis Institute for Drug Discovery
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Gastroenterology and Hepatology; Medical Genetics (excl Cancer Genetics)
URI http://hdl.handle.net/10072/46900
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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