T Helper and B Cell Escape Mutations within the HBc Gene in Patients with Asymptomatic HBV Infection: A Study From the South-Eastern Region of Iran

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Title T Helper and B Cell Escape Mutations within the HBc Gene in Patients with Asymptomatic HBV Infection: A Study From the South-Eastern Region of Iran
Author Assar, Shokrollah; Arababadi, Mohammad Kazemi; Mohit, Maryam; Ahmadabadi, Behzad Nasiri; Pumpens, Paul; Khorramdelazad, Hossein; Hajghani, Masomeh; Assar, Sepideh; Araste, Majid; Nekhei, Zohre; Sendi, Hossein; Kennedy, Derek
Journal Name Clinical Laboratory
Year Published 2012
Place of publication Germany
Publisher Clinical Laboratory Publications
Abstract Background: Escape mutations potentially allow viruses to avoid detection and clearance by the host immune sys-tem and may represent a mechanism through which infections may persist in some patients. The association of the mutations in the HBcAg gene with Hepatitis B asymptomatic carriers (ASC) has not been studied adequately. The current study was aimed to investigate HBcAg18-27 CTL epitope mutations in ASC patients in the South-Eastern region of Iran. Methods: 100 ASC patients were selected for this study and screened for HLA-A2 using flow cytometry. HBV-DNA was extracted from the HLA-A2 positive patients and the HBc gene was amplified using PCR. Direct double sequencing was performed to analyse mutations in the HBc gene of HBV isolates from patients with ASC. Results: Overall, 25 (25%) of individuals were HLA-A2 positive. Direct double sequencing indicated no mutations in the HBcAg18-27 epitope. However, four mutations within the T helper and three mutations within the B cell epitopes of ASC patients were identified. Conclusions: The lack of mutations within the HBcAg18-27 epitope suggests that the antigenicity of this region is not altered in HBV isolates of our patients and therefore antigen presentation would occur normally to the pa-tient's immune system through HLA-A2. However, in the course of this study we revealed some novel mutations within the T helper and B cell epitopes that may affect the efficiencies of immune response of ASC patients against these novel HBV epitopes. (Clin. Lab. 2012;58:53-60)
Peer Reviewed Yes
Published Yes
Publisher URI http://www.clin-lab-publications.com/issue/79
Copyright Statement Copyright 2012 Clinical Laboratory Publications. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
Volume 58
Page from 53
Page to 60
ISSN 1433-6510
Date Accessioned 2012-02-10
Language en_US
Research Centre Eskitis Institute for Drug Discovery
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Gastroenterology and Hepatology; Medical Genetics (excl Cancer Genetics)
URI http://hdl.handle.net/10072/46900
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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