Depot-specific and hypercaloric diet-induced effects on the osteoblast and adipocyte differentiation potential of adipose-derived stromal cells

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Title Depot-specific and hypercaloric diet-induced effects on the osteoblast and adipocyte differentiation potential of adipose-derived stromal cells
Author Gijsen, Hanel Sadie-Van; Smith, Wayne; Du Toit, Eugene; Michie, John; Hough, F.S.; Ferris, William Frank
Journal Name Molecular and Cellular Endocrinology
Year Published 2012
Place of publication Ireland
Publisher Elsevier
Abstract Adipose-derived stromal cells (ADSCs) can be differentiated in vitro into several mesenchyme-derived cell types. We had previously described depot-specific differences in the adipocyte differentiation of ADSCs, and consequently we hypothesized that there may also be depot-specific differences in osteoblast differentiation of ADSCs. For this study, the osteoblast differentiation potential of rat subcutaneous ADSCs (scADSCs) and perirenal visceral ADSCs (pvADSCs) was compared. Osteoblast differentiation media (OM) induced markers of the osteoblastic phenotype in scADSCs, but not in pvADSCs. ADSCs harvested from rats with diet-induced visceral obesity (DIO) exhibited reduced osteoinduction, compared to lean controls, but adipocyte differentiation was not affected. Expression of the pro-osteogenic transcription factor Msx2 was significantly higher in naïve scADSCs from lean and DIO rats than in pvADSCs. Our findings indicate that ADSCs from different anatomical sites are uniquely pre-programmed in vivo in a depot-specific manner, and that diet-induced metabolic disturbances translate into reduced osteoblast differentiation of ADSCs.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1016/j.mce.2011.07.030
Volume 348
Issue Number 1
Page from 55
Page to 66
ISSN 0303-7207
Date Accessioned 2012-06-20
Language en_US
Research Centre Griffith Health Institute
Faculty Griffith Health Faculty
Subject Cell Physiology
URI http://hdl.handle.net/10072/47325
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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