Protecting murine hearts from ischaemia-reperfusion using selective inhibitors of adenosine metabolism

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Title Protecting murine hearts from ischaemia-reperfusion using selective inhibitors of adenosine metabolism
Author Willems, Laura; Headrick, John Patrick
Journal Name Clinical and Experimental Pharmacology & Physiology
Year Published 2005
Place of publication Australia
Publisher Blackwell Publishing Asia
Abstract 1. By selectively modifying adenosine metabolism via adenosine deaminase or adenosine kinase inhibitors, it may be possible to enhance the receptor-mediated protective actions of adenosine in a site- and event-specific fashion. 2. We characterized cardioprotective actions of the adenosine deaminase inhibitor erythro-2-(2-hydroxy-3-non-yl)adenine (EHNA) and the adenosine kinase inhibitor iodotubercidin in C57/Bl6 mouse hearts subjected to 20 min global normothermic ischaemia and 40 min reperfusion. 3. Ventricular pressure development only recovered to 45 ± 2% of baseline levels (67 ± 5 mmHg) in untreated hearts, with sustained and pronounced diastolic contracture (25 ± 2 mmHg). Treatment with 20 µmol/L EHNA increased recovery of ventricular pressure (107 ± 9 mmHg), reduced postischaemic diastolic pressure (13 ± 1 mmHg) and reduced loss of lactate dehydrogenase (LDH; an indicator of necrotic damage) by 50% (9 ± 2 vs 19 ± 2 IU/g). Adenosine kinase inhibition with 10 µmol/L iodotubercidin also improved pressure development (to 100 ± 8 mmHg) and reduced LDH efflux (5 ± 2 IU/g). 4. Protective actions were mimicked by adenosine and inhibited by adenosine receptor antagonism (50 µmol/L 8-ρ-sulfophenyltheophylline) and mitochondrial KATP channel inhibition (50 µmol/L 5-hydroxydecanoate). 5. Coinfusion of the inhibitors, 'trapping' formed adenosine, failed to exert protection and, in some instances, was detrimental. Although substantial benefit was gained by these agents in hearts from young animals, neither inhibitor was effective in 'aged' hearts (18 months). 6. Our data demonstrate that adenosine deaminase or kinase inhibition substantially limits injury during ischaemia–reperfusion. Protection involves adenosine receptor activation. However, cardioprotection via either enzyme inhibitor requires an alternative purine-salvage pathway to be functional and was reduced in aged hearts known to be increasingly susceptible to ischaemic damage.
Peer Reviewed Yes
Published Yes
Alternative URI
Copyright Statement Copyright 2005 Blackwell Publishing. The definitive version is available at []
Volume 32
Issue Number 3
Page from 179
Page to 183
ISSN 0305-1870
Date Accessioned 2006-03-15
Language en_AU
Research Centre Menzies Health Institute Qld; Heart Foundation Research Centre
Faculty Griffith Health Faculty
Subject PRE2009-Basic Pharmacology
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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