Reliability and validity of conjunctival ultraviolet autofluorescence measurement

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Title Reliability and validity of conjunctival ultraviolet autofluorescence measurement
Author Sherwin, Justin C; McKnight, Charlotte M; Hewitt, Alex W; Griffiths, Lyn; Coroneo, Minas T; Mackey, David A
Journal Name British Journal of Opthalmology
Year Published 2012
Place of publication United Kingdom
Publisher BMJ Publishing Group
Abstract Background Conjunctival ultraviolet autofluorescence (UVAF) photography was developed to detect and characterise pre-clinical sunlight-induced UV damage. The reliability of this measurement and its relationship to outdoor activity are currently unknown. Methods 599 people aged 16e85 years in the crosssectional Norfolk Island Eye Study were included in the validation study. 196 UVAF individual photographs (49 people) and 60 UVAF photographs (15 people) of Norfolk Island Eye Study participants were used for intra- and inter-observer reliability assessment, respectively.Conjunctival UVAF was measured using UV photography. UVAF area was calculated using computerised methods by one grader on two occasions (intra-observer analysis) or two graders (inter-observer analysis). Outdoor activity category, during summer and winter separately, was determined with a UV questionnaire. Total UVAF equalled the area measured in four conjunctival areas (nasal/temporal conjunctiva of right and left eyes). Results Intra-observer (r_c¼0.988, 95% CI 0.967 to 0.996, p<0.001), and inter-observer concordance correlation coefficients (r_c¼0.924, 95% CI 0.870 to 0.956, p<0.001) of total UVAF exceeded 0.900. When grouped according to 10 mm2 total UVAF increments, intra- and inter-observer reliability was very good (k¼0.81) and good (k¼0.71), respectively. Increasing time outdoors was strongly with increasing total UVAF in summer and winter (ptrend <0.001). Conclusion Intra- and inter-observer reliability of conjunctival UVAF is high. In this population, UVAF correlates strongly with the authors’ survey-based assessment of time spent outdoors.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1136/bjophthalmol-2011-301255
Copyright Statement Copyright remains with the authors 2012. The attached file is reproduced here in accordance with the copyright policy of the publisher. For information about this journal please refer to the journal’s website or contact the authors.
Volume 96
Issue Number 6
Page from 801
Page to 805
ISSN 1468-2079
Date Accessioned 2012-10-18
Language en_US
Research Centre Griffith Health Institute
Faculty Griffith Health Faculty
Subject Medical and Health Sciences
URI http://hdl.handle.net/10072/47649
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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