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dc.contributor.authorGoire, Namraj
dc.contributor.authorFreeman, Kevin
dc.contributor.authorLambert, Stephen B
dc.contributor.authorNimmo, Graeme R
dc.contributor.authorLimnios, Athena E
dc.contributor.authorLahra, Monica M
dc.contributor.authorNissen, Michael D
dc.contributor.authorSloots, Theo P
dc.contributor.authorWhiley, David M
dc.date.accessioned2017-05-03T14:20:26Z
dc.date.available2017-05-03T14:20:26Z
dc.date.issued2012
dc.date.modified2013-06-17T23:05:49Z
dc.identifier.issn1448-5028
dc.identifier.doi10.1071/SH12026
dc.identifier.urihttp://hdl.handle.net/10072/47719
dc.description.abstractBackground: With treatment options for gonorrhoea (Neisseria gonorrhoeae) diminishing, strengthening antimicrobial resistance (AMR) surveillance is paramount. Methods: In this study, we investigated polymerase chain reaction (PCR) based methods, in parallel with N. gonorrhoeae multi-antigen sequence typing (NG-MAST), for direct detection of four N. gonorrhoeae chromosomal mechanisms associated with emerging resistance to extended spectrum cephalosporins using noncultured samples: an adenine deletion in the mtrR promoter, a mosaic penicillin-binding protein (PBP) 2, an A501V PBP2 mutation, and alterations at positions 120 and 121 of the porB protein. The PCR assays were validated using a panel of characterised N. gonorrhoeae isolates (n = 107) and commensal Neisseria (n = 100) species. These PCR assays with NG-MAST were then applied to noncultured clinical specimens from distinct populations in Australia with differing levels of N. gonorrhoeae AMR: the Northern Territory (NT), where resistance has a low population prevalence, and Queensland (Qld), with higher AMR prevalence. Results: The real-time PCR assays proved highly sensitive and specific. When applied to the noncultured samples, only 1 out of 50 (2%) samples from NT harboured a resistant mechanism, whereas the Qld samples (n = 129) collected over different periods showed progressive acquisition of resistant mechanisms, and these were associated with specific NG-MAST types, including Type 225. Conclusions: The results suggest that our PCR-based methods could be used to rapidly pinpoint incursion of resistant strains into previously unaffected populations. Likewise, our results show that for molecular AMR surveillance, the population being investigated is as important as the genetic mechanisms being targeted.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherCSIRO Publishing
dc.publisher.placeAustralia
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom422
dc.relation.ispartofpageto429
dc.relation.ispartofissue5
dc.relation.ispartofjournalSexual Health
dc.relation.ispartofvolume9
dc.rights.retentionY
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchHuman society
dc.subject.fieldofresearchcode32
dc.subject.fieldofresearchcode44
dc.titleThe influence of target population on nonculture-based detection of markers of Neisseria gonorrhoeae antimicrobial resistance
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2012
gro.hasfulltextNo Full Text
gro.griffith.authorNimmo, Graeme R.


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