The human stress-activated protein kinase-interacting 1 gene encodes JNK-binding proteins.

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Title The human stress-activated protein kinase-interacting 1 gene encodes JNK-binding proteins.
Author Schroder, Wayne Ashley; Bushell, Gillian Robin; Sculley, Tom
Journal Name Cellular Signalling
Year Published 2005
Place of publication USA
Publisher Elsevier
Abstract The orthologous proteins of the stress-activated protein kinase-interacting 1 (Sin1) family have been implicated in several different signal transduction pathways. In this study, we have investigated the function of the full-length human Sin1 protein and a C-terminally truncated isoform, Sin1a, which is produced by alternative splicing. Immunoblot analysis using an anti-Sin1 polyclonal antibody showed that full-length Sin1 and several smaller isoforms are widely expressed. Sin1 was demonstrated to bind to c-Jun N-terminal kinase (JNK) in vitro and in vivo, while no interaction with p38- or ERK1/2-family MAPKs was observed. The Sin1a isoform could also form a complex with JNK in vivo. Despite localizing in distinct compartments within the cell, both Sin1 and Sin1a co-localized with JNK, suggesting that the Sin1 proteins could recruit JNK. Over-expression of full-length Sin1 inhibited the activation of JNK by UV-C in DG75 cells, as well as basal JNK-activity in HEK293 cells. These data suggest that the human Sin1 proteins may act as scaffold molecules in the regulation of signaling by JNK.
Peer Reviewed Yes
Published Yes
Publisher URI http://www.elsevier.com/wps/find/journaldescription.cws_home/525462/description#description
Alternative URI http://dx.doi.org/10.1016/j.cellsig.2004.10.015
Copyright Statement Copyright 2002 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links
Volume 17
Issue Number 6
Page from 761
Page to 767
ISSN 0898-6568
Date Accessioned 2006-01-12
Language en_AU
Faculty Faculty of Science
Subject PRE2009-Protein Targeting and Signal Transduction
URI http://hdl.handle.net/10072/4979
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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