ßA- and ßC-activin, follistatin, activin receptor mRNA and ßC-activin peptide expression during rat liver regeneration

Abstract

The mRNA expression of two activin growth factor subunits (ßA- and ßC-activin), activin receptor subunits (ActRIIA, ActRIIB) and the activin-binding protein follistatin, and peptide expression of ßA-activin and ßC-activin subunits, were examined in regenerating rat liver after partial hepatectomy (PHx). Liver samples were collected from adult, male SpragueDawley rats, 12240 h (n=35 rats per time point) after PHx or from sham-operated controls at the same time points. Hepatocyte mitosis and apoptosis were assessed histologically and by in situ cell death detection. RT and PCR were used to assess relative gene expression. ßA- and ßC-activin peptide immunoreactivity was assessed in liver and serum samples by western blotting, whereas cellular expression was investigated by immunohistochemistry, using specific monoclonal antibodies. ßA- and ßC-activin mRNA dropped to < 50% of sham control values 12 h after PHx and remained at this level until 168 h post-PHx, when ßA-activin expression increased to three times sham control values and ßC-activin mRNA returned to pre-PHx levels. A peak in follistatin expression was observed 2448 h post-PHx, coincident with an increase in hepatocyte mitosis. No changes were observed in ActRIIA mRNA, whereas ActRIIB expression paralleled that of ßA-activin mRNA. ßC-activin immunoreactive homo- and heterodimers were observed in regenerating liver and serum. Mitotic hepatocytes frequently contained ßC-activin immunoreactivity, whereas apoptotic hepatocytes were often immunoreactive for ßA-activin. We conclude that ßA- and ßC-activin subunit proteins are autocrine growth regulators in regenerating liver and when expressed independently lead to hepatocyte apoptosis or mitosis in a subset of hepatocytes