Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei

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Title Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei
Author Holden, Matthew T. G.; Titball, Richard W.; Peacock, Sharon J.; Cerdeno-Tarraga, Ana M.; Atkins, Timothy; Crossman, Lisa C.; Pitt, Tyrone; Churcher, Carol; Mungall, Karen; Bentley, Stephen D.; Sebaihia, Mohammed; Thomson, Nicholas R.; Bason, Nathalie; Beacham, Ifor Rhys; Brooks, Karen; Brown, Katherine A.; Brown, Nat; Challis, Greg L.; Cherevach, Inna; Chillingworth, Tracy; Cronin, Ann; Crosset, Ben; Davis, Paul; DeShazer, David; Feltwell, Theresa; Fraser, Audrey; Hance, Zahra; Hauser, Heidi; Holroyd, Simon; Jagels, Kay; Keith, Karen E.; Maddison, Mark; Moule, Sharon; Price, Claire; Quail, Michael A.; Rabbinowitsch, Ester; Rutherford, Kim; Sanders, Mandy; Simmonds, Mark; Songsivilai, Sirirunrg; Stevens, Kim; Tumapa, Sarinna; Vesaratchavest, Mokgol; Whitehead, Sally; Yeats, Corin; Barrell, Bart G.; Oyston, Petra; Parkhill, Julian
Journal Name Proceedings of the National Acadmey of Sciences (USA)
Editor N. R. Cozzarelli
Year Published 2004
Place of publication USA
Publisher National Academy of Sciences
Abstract Burkholderia pseudomallei is a recognized biothreat agent and the causative agent of melioidosis. This Gram-negative bacterium exists as a soil saprophyte in melioidosis-endemic areas of the world and accounts for 20% of community-acquired septicaemias in northeastern Thailand where half of those affected die. Here we report the complete genome of B. pseudomallei, which is composed of two chromosomes of 4.07 megabase pairs and 3.17 megabase pairs, showing significant functional partitioning of genes between them. The large chromosome encodes many of the core functions associated with central metabolism and cell growth, whereas the small chromosome carries more accessory functions associated with adaptation and survival in different niches. Genomic comparisons with closely and more distantly related bacteria revealed a greater level of gene order conservation and a greater number of orthologous genes on the large chromosome, suggesting that the two replicons have distinct evolutionary origins. A striking feature of the genome was the presence of 16 genomic islands (GIs) that together made up 6.1% of the genome. Further analysis revealed these islands to be variably present in a collection of invasive and soil isolates but entirely absent from the clonally related organism B. mallei. We propose that variable horizontal gene acquisition by B. pseudomallei is an important feature of recent genetic evolution and that this has resulted in a genetically diverse pathogenic species.
Peer Reviewed Yes
Published Yes
Publisher URI
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Volume 101
Issue Number 39
Page from 14240
Page to 14245
ISSN 0027-8424
Date Accessioned 2005-03-04
Language en_AU
Faculty Institute for Glycomics
Subject PRE2009-Genome Structure
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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