Age-related changes in cardiac adenosine receptor expression

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Title Age-related changes in cardiac adenosine receptor expression
Author Jenner, Tamsin; Mellick, Albert; Harrison, Glenn; Griffiths, Lyn; Rose'Meyer, Roselyn
Journal Name Mechansims of Ageing and Development
Year Published 2004
Place of publication Ireland
Publisher Elsevier
Abstract Adenosine is an important cardioprotective agent that works via several adenosine receptor (ADOR) subtypes to regulate cardiovascular activity. It is well established that functional responses to adenosine decline with age. What is unclear, though, is whether these changes occur at the receptor, second messenger or translational level. In this study we determined the effect of age on cardiac adenosine receptor expression using the housekeeping gene 18S rRNA versus the adenosine A2B receptor gene as internal controls. Absolute quantification showed that no age-related changes occurred in the expression of 18S rRNA or adenosine A2B receptor internal control genes. Subsequently, relative analysis of the adenosine receptor subtypes using 18S rRNA found a significant age-related reduction in the expression of the adenosine A1 receptor (5.5-fold), with no changes in the expression of the adenosine A2A, A2B and A3 receptors. When using the expression of the adenosine A2B receptor as the internal control gene, a significant down regulation of both the adenosine A1 (5.4-fold) and A2A (2.2-fold) receptors with no change in the expression of adenosine A3 receptor was found. Therefore, the high level of expression of the 18S rRNA housekeeping gene was found to mask a significant change in expression of the adenosine A2A receptor with age. Ultimately, these findings show an age-related reduction in adenosine A1 and A2A receptor expression in rat heart.
Peer Reviewed Yes
Published Yes
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Copyright Statement Copyright 2004 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links.
Volume 125
Page from 211
Page to 217
ISSN 0047-6374
Date Accessioned 2005-01-12
Language en_AU
Research Centre Menzies Health Institute Qld; Heart Foundation Research Centre
Faculty Griffith Health Faculty
Subject Medical & Health Sciences
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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