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dc.contributor.authorJenner, TL
dc.contributor.authorMellick, AS
dc.contributor.authorHarrison, GJ
dc.contributor.authorGriffiths, LR
dc.contributor.authorRose'Meyer, RB
dc.date.accessioned2017-05-03T14:05:55Z
dc.date.available2017-05-03T14:05:55Z
dc.date.issued2004
dc.date.modified2008-11-30T22:45:37Z
dc.identifier.issn0047-6374
dc.identifier.doi10.1016/j.mad.2003.11.016
dc.identifier.urihttp://hdl.handle.net/10072/5645
dc.description.abstractAdenosine is an important cardioprotective agent that works via several adenosine receptor (ADOR) subtypes to regulate cardiovascular activity. It is well established that functional responses to adenosine decline with age. What is unclear, though, is whether these changes occur at the receptor, second messenger or translational level. In this study we determined the effect of age on cardiac adenosine receptor expression using the housekeeping gene 18S rRNA versus the adenosine A2B receptor gene as internal controls. Absolute quantification showed that no age-related changes occurred in the expression of 18S rRNA or adenosine A2B receptor internal control genes. Subsequently, relative analysis of the adenosine receptor subtypes using 18S rRNA found a significant age-related reduction in the expression of the adenosine A1 receptor (5.5-fold), with no changes in the expression of the adenosine A2A, A2B and A3 receptors. When using the expression of the adenosine A2B receptor as the internal control gene, a significant down regulation of both the adenosine A1 (5.4-fold) and A2A (2.2-fold) receptors with no change in the expression of adenosine A3 receptor was found. Therefore, the high level of expression of the 18S rRNA housekeeping gene was found to mask a significant change in expression of the adenosine A2A receptor with age. Ultimately, these findings show an age-related reduction in adenosine A1 and A2A receptor expression in rat heart.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeIreland
dc.publisher.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/506026/description#description
dc.relation.ispartofpagefrom211
dc.relation.ispartofpageto217
dc.relation.ispartofjournalMechansims of Ageing and Development
dc.relation.ispartofvolume125
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode3101
dc.titleAge-related changes in cardiac adenosine receptor expression
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medical Science
gro.rights.copyright© 2004 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links.
gro.date.issued2004
gro.hasfulltextNo Full Text
gro.griffith.authorHarrison, Glenn J.
gro.griffith.authorRose'Meyer, Roselyn B.


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