Effects of dietary selenium on glutathione peroxidase and thioredoxin reductase activity and recovery from cardiac ischemia-reperfusion

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Title Effects of dietary selenium on glutathione peroxidase and thioredoxin reductase activity and recovery from cardiac ischemia-reperfusion
Author Venardos, Kylie; Harrison, Glenn; Headrick, John Patrick; Perkins, Anthony Vincent
Journal Name Journal of Trace Elements in Medicine and Biology
Editor Virginia Negretti de Braetter [Editor-in-Chief]
Year Published 2004
Place of publication Germany
Publisher Elsevier GmbH, Urban & Fischer Verlag
Abstract Glutathione peroxidase and thioredoxin reductase are selenocysteine-dependent enzymes that protect against oxidative injury. This study examined the effects of dietary selenium on the activity of these two enzymes in rats, and investigated the ability of selenium to modulate myocardial function post ischemia–reperfusion. Male wistar rats were fed diets containing 0, 50, 240 and 1000 μg/kg sodium selenite for 5 weeks. Langendorff perfused hearts isolated from these rats were subjected to 22.5 min global ischemia and 45 min reperfusion, with functional recovery assessed. Liver samples were collected at the time of sacrifice, and heart and liver tissues assayed for thioredoxin reductase and glutathione peroxidase activity. Selenium deficiency reduced the activity of both glutathione peroxidase and thioredoxin reductase systemically. Hearts from selenium deficient animals were more susceptible to ischemia–reperfusion injury when compared to normal controls (38% recovery of rate pressure product (RPP) vs. 47% recovery of RPP). Selenium supplementation increased the endogenous activity of thioredoxin reductase and glutathione peroxidase and resulted in improved recovery of cardiac function post ischemia–reperfusion (57% recovery of RPP). Endogenous activity of glutathione peroxidase and thioredoxin reductase is dependent on an adequate supply of the micronutrient selenium. Reduced activity of these antioxidant enzymes is associated with significant reductions in myocardial function post ischemia–reperfusion.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1016/j.jtemb.2004.01.001
Copyright Statement Copyright 2004 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online
Volume 18
Issue Number 1
Page from 81
Page to 88
ISSN 0946-672X
Date Accessioned 2005-04-06
Language en_AU
Research Centre Griffith Health Institute; Heart Foundation Research Centre
Faculty Griffith Health Faculty
Subject PRE2009-Biochemistry and Cell Biology; PRE2009-Biological and Medical Chemistry
URI http://hdl.handle.net/10072/5663
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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