Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue

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Title Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue
Author Smith, Robert A; Lea, Rod A; Curran, Joanne E; Weinstein, Stephen R; Griffiths, Lyn R
Journal Name Breast Cancer Research
Editor Bruce AJ Ponder
Year Published 2003
Place of publication United Kingdom
Publisher BioMed Central
Abstract Background Previous studies in our laboratory have shown associations of specific nuclear receptor gene variants with sporadic breast cancer. In order to investigate these findings further, we conducted the present study to determine whether expression levels of the progesterone and glucocorticoid nuclear receptor genes vary in different breast cancer grades. Methods RNA was extracted from paraffin-embedded archival breast tumour tissue and converted into cDNA. Sample cDNA underwent PCR using labelled primers to enable quantitation of mRNA expression. Expression data were normalized against the 18S ribosomal gene multiplex and analyzed using analysis of variance. Results Analysis of variance indicated a variable level of expression of both genes with regard to breast cancer grade (P = 0.00033 for glucocorticoid receptor and P = 0.023 for progesterone receptor). Conclusion Statistical analysis indicated that expression of the progesterone nuclear receptor is elevated in late grade breast cancer tissue.
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Copyright Statement Copyright 2003 Smith et al., licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Volume 5
Issue Number 1
Page from R9
Page to R12
ISSN 1465-5411
Date Accessioned 2004-03-31
Language en_AU
Research Centre Menzies Health Institute Qld; Molecular Basis of Disease
Faculty Griffith Health Faculty
Subject PRE2009-Gene Expression
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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