Sequence analysis of a cryptic plasmid pCJ419 from Campylobacter jejuni and construction of an Escherichia coli-Campylobacter shuttle vector.

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Title Sequence analysis of a cryptic plasmid pCJ419 from Campylobacter jejuni and construction of an Escherichia coli-Campylobacter shuttle vector.
Author Alfredson, David; Korolik, Victoria
Journal Name Plasmid
Editor D. K. Chattoraj
Year Published 2003
Place of publication San Diego, USA
Publisher Academic Press inc. Elsevier Science
Abstract A small cryptic plasmid, pCJ419, was identified in a human clinical isolate of Campylobacter jejuni, cloned and sequenced. pCJ419 is a circular molecule of 4013 bp with a G + C content of 27.1%. The products of four open reading frames (ORFs) share significant sequence similarity with putative proteins from known C. jejuni and Campylobacter coli plasmids. ORF-1 encodes a putative mobilisation protein (Mob). ORF-2 and ORF-3 encode proteins that have high identity to putative RepA and RepB proteins, respectively, of known C. jejuni and C. coli plasmids. ORF-4 encodes a protein that has high identity to a hypothetical protein of unknown function, Cjp32, previously described in a pVir plasmid of C. jejuni. Tandem repeating 22-bp sequences typical of a plasmid replication origin (ori) were identified upstream of the DNA sequences encoding putative replication initiation proteins. An Escherichia coli–Campylobacter shuttle cloning vector, pGU0202, was constructed using plasmid pMW2 that harbours a Campylobacter-derived kanamycin resistance gene [aph(3′)-III]. The sequences encoding pCJ419 mob, RepA and RepB proteins were inserted upstream of aph(3′)-III resulting in a stable construct of 6174 bp that was used to transform both E. coli and Campylobacter.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1016/S0147-619X(03)00060-X
Volume 50
Page from 152
Page to 160
ISSN 0147-619X
Date Accessioned 2004-03-31
Date Available 2015-05-06T21:36:40Z
Language en_US
Research Centre Institute for Glycomics
Faculty Faculty of Nursing & Health
Subject PRE2009-Bacteriology
URI http://hdl.handle.net/10072/6230
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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