Antimicrobial resistance and genomic screening of clinical isolates of thermophilic Campylobacter spp. from south-east Queensland, Australia

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Title Antimicrobial resistance and genomic screening of clinical isolates of thermophilic Campylobacter spp. from south-east Queensland, Australia
Author Alfredson, David; Akhurst, R.J.; Korolik, Victoria
Journal Name Journal of Applied Microbiology
Editor Duncan E S Stewart Tull
Year Published 2003
Place of publication Oxford, England
Publisher Blackwell Publishing Ltd
Abstract Aim: To screen 90 clinical isolates of thermophilic Campylobacter species for putative resistance to ampicillin, erythromycin and tetracycline and perform numerical analysis to determine isolate relatedness. Methods and Results: Disc diffusion, E-test MIC and agar dilution methods were performed. Disc diffusion testing showed 87 (97%) isolates appeared resistant to ampicillin at 10 ¼g; 14 (16%) resistant to tetracycline at 30 ¼g; and three (3·4%) resistant to erythromycin at 15 ¼g. E-test MICs showed a range of 0·5 to >256 mg l1 for ampicillin; 16 to >256 mg l1 for tetracycline; and >256 mg l1 for erythromycin. E-test showed 68% correlation (±1 log2 dilution) with agar dilution for ampicillin, 100% for erythromycin and 64% for tetracycline. Disc diffusion testing showed 100% correlation with agar dilution for erythromycin and tetracycline, and 77% for ampicillin. Numerical analyses of restriction endonuclease (RE) fragment profiles suggested a high level of isolate variation. Conclusion: The incidence of resistance of thermophilic Campylobacter spp. to erythromycin and tetracycline is low in south-east Queensland. Significance and Impact of the Study: Disc diffusion susceptibility testing may be used to screen thermophilic Campylobacter spp. for putative resistance to erythromycin and tetracycline. Agar dilution should be used to determine ampicillin susceptibility.
Peer Reviewed Yes
Published Yes
Publisher URI http://www.blackwell-synergy.com/doi/full/10.1046/j.1365-2672.2003.01856.x
Alternative URI http://www.blackwell-synergy.com/links/doi/10.1046%2Fj.1365-2672.2003.01856.x
Copyright Statement Copyright 2003 Blackwell Publishing. The definitive version is available at [www.blackwell-synergy.com.]
Volume 94
Page from 495
Page to 500
ISSN 1364-5072
Date Accessioned 2004-03-31
Language en_AU
Research Centre Institute for Glycomics
Faculty Faculty of Nursing & Health
Subject Bacteriology
URI http://hdl.handle.net/10072/6232
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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