Molecular and Genomic Analysis of Genes Encoding Surface-Anchored Proteins from Clostridium difficile
Author(s)
Karjalainen, T
Waligora-Dupriet, AJ
Cerquetti, M
Spigaglia, P
Maggioni, A
Mauri, P
Mastrantonio, P
Griffith University Author(s)
Year published
2001
Metadata
Show full item recordAbstract
The gene slpA, encoding the S-layer precursor protein in the virulent Clostridium difficile strains C253 and 79–685, was identified. The precursor protein carries a C-terminal highly conserved anchoring domain, similar to the one found in the Cwp66 adhesin (previously characterized in strain 79–685), an SLH domain, and a variable N-terminal domain mediating cell adherence. The genes encoding the S-layer precursor proteins and the Cwp66 adhesin are present in a genetic locus carrying 17 open reading frames, 11 of which encode a similar two-domain architecture, likely to include surface-anchored proteins.The gene slpA, encoding the S-layer precursor protein in the virulent Clostridium difficile strains C253 and 79–685, was identified. The precursor protein carries a C-terminal highly conserved anchoring domain, similar to the one found in the Cwp66 adhesin (previously characterized in strain 79–685), an SLH domain, and a variable N-terminal domain mediating cell adherence. The genes encoding the S-layer precursor proteins and the Cwp66 adhesin are present in a genetic locus carrying 17 open reading frames, 11 of which encode a similar two-domain architecture, likely to include surface-anchored proteins.
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Journal Title
Infection and Immunity
Volume
69
Issue
5
Subject
Biological sciences
Genomics
Agricultural, veterinary and food sciences
Biomedical and clinical sciences