Laboratory validation of enzyme-immunoassays for the non-invasive quantification of reproductive and stress hormone in amphibians
Author(s)
Narayan, Edward Jitik
Griffith University Author(s)
Year published
2014
Metadata
Show full item recordAbstract
Quantification of reproductive and stress hormones entirely non-invasively using amphibian urine samples has provided invaluable tool for understanding the biological functioning of reproductive and stress endocrine axis in rare, endangered and threatened species. This technical paper, I describe step by step methods for the laboratory validation of enzyme-immunoassays (EIAs) for the quantification of reproductive hormones (testosterone, oestradiol and progesterone) and stress hormone (corticosterone) entirely non-invasively in amphibian urine samples. These laboratory validation steps include (1) Accuracy/recovery checks, ...
View more >Quantification of reproductive and stress hormones entirely non-invasively using amphibian urine samples has provided invaluable tool for understanding the biological functioning of reproductive and stress endocrine axis in rare, endangered and threatened species. This technical paper, I describe step by step methods for the laboratory validation of enzyme-immunoassays (EIAs) for the quantification of reproductive hormones (testosterone, oestradiol and progesterone) and stress hormone (corticosterone) entirely non-invasively in amphibian urine samples. These laboratory validation steps include (1) Accuracy/recovery checks, (2) Parallelism and (3) Quality control. I also present standard assay methods and together these will enable the advancement of non-invasive reproductive and stress endocrinology of amphibians.
View less >
View more >Quantification of reproductive and stress hormones entirely non-invasively using amphibian urine samples has provided invaluable tool for understanding the biological functioning of reproductive and stress endocrine axis in rare, endangered and threatened species. This technical paper, I describe step by step methods for the laboratory validation of enzyme-immunoassays (EIAs) for the quantification of reproductive hormones (testosterone, oestradiol and progesterone) and stress hormone (corticosterone) entirely non-invasively in amphibian urine samples. These laboratory validation steps include (1) Accuracy/recovery checks, (2) Parallelism and (3) Quality control. I also present standard assay methods and together these will enable the advancement of non-invasive reproductive and stress endocrinology of amphibians.
View less >
Journal Title
Protocol Exchange
Volume
n/a
Subject
Analytical Biochemistry
Comparative Physiology