Purification and Characterization of an N-Acetyl-D-Glucosamine Specific Lectin from the Australian Mushroom Psathyrella Asperospora
Author(s)
Abol Hassan, Ali
Rouf, Razina
Pribeiro, Joao
Jaggin, Fabia Valentina
Day, Christopher
Varrot, Annabelle
Tiralongo, Evelin
Way, Tom
Imberty, Anne
Tiralongo, Joe
Griffith University Author(s)
Year published
2014
Metadata
Show full item recordAbstract
Australia has a spectacular biodiversity including animals, plants and fungi. Indigenous Australians have been using higher
fungi traditionally as medicines and in religious practice for thousands of years. It has been estimated that there is a large
number of different mushroom species present in Australia that are poorly explored and catalogued. Importantly, very little
is known about the extent and diversity of lectins from Australian mushroom species [1]. Psathyrella asperospora
(Family: Psathyrellaceae) (synonym Lacrymaria asperospora) is an Australian indigenous mushroom from which we have
isolated an N-acetyl-D-glucosamine ...
View more >Australia has a spectacular biodiversity including animals, plants and fungi. Indigenous Australians have been using higher fungi traditionally as medicines and in religious practice for thousands of years. It has been estimated that there is a large number of different mushroom species present in Australia that are poorly explored and catalogued. Importantly, very little is known about the extent and diversity of lectins from Australian mushroom species [1]. Psathyrella asperospora (Family: Psathyrellaceae) (synonym Lacrymaria asperospora) is an Australian indigenous mushroom from which we have isolated an N-acetyl-D-glucosamine (GlcNAc) specific lectin [1]. De novo sequencing of Psathyrella asperospora lectin (PAL) using LC-MS/MS, identified 10 tryptic peptides that revealed substantial sequence similarity to the GlcNAc specific lectin from Psathyrella velutina (PVL) in both carbohydrate binding and calcium binding sites. Significantly, we also found that PAL has anti-proliferative effect on human colon cancer HT29 cells with an IC50 of 0.48 μM that represents one of the most potent mushroom lectin yet reported [2]. Further characterization of PAL’s anti-proliferative activity using propidium iodine staining revealed that it induced cell cycle arrest at G2/M phase in a manner dependent on its ability to bind GlcNAc on the cell surface. Large scale purification of PAL has now been performed in order to fully characterise the carbohydrate binding specificity including its thermodynamic properties and structural determination using glycan arrays, isothermal calorimetry (ITC) and X-ray crystallography.
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View more >Australia has a spectacular biodiversity including animals, plants and fungi. Indigenous Australians have been using higher fungi traditionally as medicines and in religious practice for thousands of years. It has been estimated that there is a large number of different mushroom species present in Australia that are poorly explored and catalogued. Importantly, very little is known about the extent and diversity of lectins from Australian mushroom species [1]. Psathyrella asperospora (Family: Psathyrellaceae) (synonym Lacrymaria asperospora) is an Australian indigenous mushroom from which we have isolated an N-acetyl-D-glucosamine (GlcNAc) specific lectin [1]. De novo sequencing of Psathyrella asperospora lectin (PAL) using LC-MS/MS, identified 10 tryptic peptides that revealed substantial sequence similarity to the GlcNAc specific lectin from Psathyrella velutina (PVL) in both carbohydrate binding and calcium binding sites. Significantly, we also found that PAL has anti-proliferative effect on human colon cancer HT29 cells with an IC50 of 0.48 μM that represents one of the most potent mushroom lectin yet reported [2]. Further characterization of PAL’s anti-proliferative activity using propidium iodine staining revealed that it induced cell cycle arrest at G2/M phase in a manner dependent on its ability to bind GlcNAc on the cell surface. Large scale purification of PAL has now been performed in order to fully characterise the carbohydrate binding specificity including its thermodynamic properties and structural determination using glycan arrays, isothermal calorimetry (ITC) and X-ray crystallography.
View less >
Conference Title
8th International Conference on Mushroom Biology and Mushroom Products (ICMBMP8) 2014
Publisher URI
Subject
Science, Technology and Engineering Curriculum and Pedagogy